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Test ID BTWGS Bacterial Typing by Whole Genome Sequencing

Biohazard Infectious

Useful For

Aids in investigation of a potential outbreak by a single bacterial species


May assist in identification of recurrent infection in an individual patient

Method Name

Whole Genome Sequencing

Reporting Name

Bacterial Typing, Whole Genome Seq

Specimen Type


Advisory Information

Currently only Staphylococcus aureus is being tested by this method. For other bacteria, order PFGE / Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE). PFGE orders for S aureus will be automatically converted to BTWGS / Bacterial Strain Typing by Whole Genome Sequencing.

Shipping Instructions

1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place all isolates submitted for whole genome sequencing (WGS) together in one large bag, and send it in the same shipping container. This is necessary for comparison of isolates by this method.

Necessary Information

1. Organism identification and specimen source are required for processing.

2. Each slant must be submitted under a separate order.

3. An email address registered on the Mayo Medical Laboratories website is required for report delivery.

Specimen Required

Supplies: Infectious Container, Large (T146)

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants in pure culture

Collection Instructions:

1. Isolate the bacteria (must be Staphylococcus aureus)

2. Bacterial organism must be in pure culture, actively growing. Do not submit mixed cultures.

3. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)

Reference Values

Not applicable

Day(s) and Time(s) Performed

Varies; Batched 1 time per week

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

0010U-Bacterial Typing, Whole Genome Seq

87900-Bioinformatics Reanalysis (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BTWGS Bacterial Typing, Whole Genome Seq In Process


Result ID Test Result Name Result LOINC Value
BTWGS Bacterial Typing, Whole Genome Seq In Process

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
BIORE Bioinformatics Reanalysis No, (Bill Only) No

Testing Algorithm

When reanalysis of previously submitted isolates for comparison to new isolates is requested, BIORE / Bioinformatics Reanalysis (Bill Only) will be added by Mayo Medical Laboratories. Original patient submission information (names and order numbers) must be provided.