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Test ID HELIS Helicobacter pylori Culture with Antimicrobial Susceptibilities

Useful For

Recovery of Helicobacter pylori from gastric specimens for antimicrobial susceptibility testing of the organism (amoxicillin, ciprofloxacin, clarithromycin, metronidazole and tetracycline are routinely tested)

Method Name

Conventional Culture Techniques

Reporting Name

Helicobacter pylori Culture + Susc

Specimen Type

Varies

Specimen Required

Preferred:

Specimen Type: Gastric biopsy

Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions: Acquire biopsied tissue; moisten with sterile saline.

 

Acceptable:

Specimen Type: Gastric brushings or gastric aspirate

Container/Tube: Sterile container

Specimen Volume: Entire collection

Specimen Minimum Volume

0.5 mL or 0.5 × 0.2 × 0.2-cm sized piece of tissue

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated 48 hours

Reference Values

No growth after 7 days

Susceptibility results are reported as minimum inhibitory concentration (MIC) in mcg/mL and as susceptible, intermediate, or resistant according to the Clinical and Laboratory Standards Institute (CLSI) guidelines.

 

In some instances an interpretive category cannot be provided based on available data and the following comment will be included: "There are no established interpretive guidelines for agents reported without interpretations."

 

Susceptible (S):

The "susceptible" category implies that isolates are inhibited by the usually achievable concentrations of antimicrobial agent when the dosage recommended to treat the site of infection is used, resulting in likely clinical efficacy.

Intermediate (I)

The "intermediate" category includes isolates with antimicrobial agent minimum inhibitory concentrations (MICs) that approach usually attainable blood and tissue levels, and for which response rates may be lower than for susceptible isolates.

Note: The intermediate category implies clinical efficacy in body sites where the drugs are physiologically concentrated or when a higher than normal dosage of a drug can be used. This category also includes a buffer zone, which should prevent small, uncontrolled, technical factors from causing major discrepancies in interpretations, especially for drugs with narrow pharmacotoxicity margins.

Resistant (R)

The "resistant" category implies that the isolates are not inhibited by the usually achievable concentrations of the agent with normal dosage schedules and/or that demonstrate MIC that fall in the range where specific microbial resistance mechanisms are likely, and clinical efficacy of the agent against the isolate has not been reliably shown in treatment studies.

(Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing. 26th Informational Supplement. CLSI document M100S. Wayne, PA, 2016)

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Performing Laboratory

Mayo Medical Laboratories in Rochester

CPT Code Information

87081-Helicobacter pylori culture

87077-Bacteria identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87176-Tissue processing (if appropriate)

87181-Susceptibility (if appropriate)

87186-Sensitivity, MIC (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HELIS Helicobacter pylori Culture + Susc 587-6

 

Result ID Test Result Name Result LOINC Value
HELIS Helicobacter pylori Culture + Susc 587-6

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged.

 

When Helicobacter pylori is isolated, identification will be confirmed and susceptibility testing performed. The routine susceptibility panel includes amoxicillin, ciprofloxacin, clarithromycin, metronidazole, and tetracycline.

 

See Helicobacter pylori Diagnostic Algorithm in Special Instructions

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
TISSR Tissue Processing No, (BIll Only) No
MIC Sensitivity, MIC No, (Bill Only) No
SUS Susceptibility No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

Necessary Information

Specimen source is required; include the specific anatomic source.

Shipping Instructions

Specimen must be received in laboratory within 48 hours of collection.