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Test ID JAKXM JAK2 Exon 12 and Other Non-V617F Mutation Detection, Bone Marrow

Useful For

Aiding in the distinction between a reactive cytosis and a myeloproliferative neoplasm, particularly when a diagnosis of polycythemia is being entertained; for use with bone marrow specimens

Method Name

Mutation Detection in cDNA Using Sanger Sequencing
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Reporting Name

JAK2 Exon 12 Mutation Detection, BM

Specimen Type

Bone Marrow

Specimen Required

Container/Tube:

Preferred: EDTA (lavender top)

Acceptable: ACD (yellow top)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.


Shipping Instructions:

Specimen must arrive within 5 days (120 hours) of collection. Draw and package specimen as close to shipping time as possible.



Necessary Information:

Date of collection is required.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Bone Marrow Refrigerated (preferred) 5 days
  Ambient  5 days

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Medical Laboratories in Rochester

CPT Code Information

81403-JAK2 (Janus kinase 2) (eg, myeloproliferative disorder), exon 12 sequence and exon 13 sequence, if performed

LOINC Code Information

Test ID Test Order Name Order LOINC Value
JAKXM JAK2 Exon 12 Mutation Detection, BM In Process

 

Result ID Test Result Name Result LOINC Value
39468 JAK2 Sequencing Result In Process
20250 Final Diagnosis: 34574-4

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Testing Algorithm

This is a second-order test that should be used when the test for the JAK2M / JAK2 V617F Mutation Detection, Bone Marrow test is negative.

 

See Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation in Special Instructions.

Forms

1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request Form (T726) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/hematopathology-request-form.pdf).