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Test ID RASFP RAS/RAF Targeted Gene Panel by Next-Generation Sequencing, Tumor

Useful For

Identifying tumors that may respond to targeted therapies by assessing multiple gene targets simultaneously

 

Identifying mutations that may help determine prognosis for patients with solid tumors

 

Identifying specific mutations within genes known to be associated with response or resistance to specific cancer therapies

Genetics Test Information

This extended RAS/RAF panel test uses targeted next-generation sequencing to evaluate for somatic mutations within the BRAF (exons 11 and 15), HRAS (exons 2 and 3), NRAS (exons 2, 3, 4), and KRAS (exons 2, 3, 4) genes. This includes, but is not limited to, the testing of somatic mutations in KRAS codons 12, 13, 59, 61, 117, 146; NRAS codons 12, 13, 59, 61,146; HRAS codons 12, 13, 61; and BRAF codons 594, 596, 600. See Targeted Gene Regions Interrogated by RAS/RAF Gene Panel in Special Instructions for details regarding the targeted gene regions identified by this test.

 

Of note, this test is performed to evaluate for somatic mutations within tumor samples. This test does not assess for germline alterations within the genes listed.

Highlights

This test provides evaluation of BRAF (including V600), HRAS, NRAS (includes codons 12, 13, 59, 61, and 146), and KRAS (includes codons 12, 13, 59, 61, 117, and 146) genes for somatic mutations.

Additional Tests

Test ID Reporting Name Available Separately Always Performed
SLIRV Slide Review in MG No Yes

Testing Algorithm

When this test is ordered, slide review will always be performed at an additional charge.

Method Name

Polymerase Chain Reaction (PCR)-Based Next Generation Sequencing

Reporting Name

RAS/RAF Panel, Tumor

Specimen Type

Varies


Advisory Information


At least 20% tumor is required for this assay. The amount of tissue needed is dependent on a variety of preanalytical factors (eg, cellularity, ischemic time, fixation). In general, the minimum specimen adequacy for this test is approximately a 6 mm(2) area of tissue (can be over multiple slides from one tissue block) or 5,000 total cells (5,000 total nucleated cells if using cytology slides).



Necessary Information


A pathology report must accompany specimen in order for testing to be performed.



Specimen Required


Preferred:

Specimen Type: Tissue

Container/Tube: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block.

 

Acceptable:

Specimen Type: Tissue

Slides: 1 stained and 10 unstained

Collection Instructions: Submit 1 slide stained with hematoxylin and eosin and 10 unstained, unbaked slides with 5-micron thick sections of the tumor tissue.

 

Acceptable:

Specimen Type: Cytology

Container/Tube: Cytology slide (Direct smears or ThinPrep)

Slides: 1-2 slides (stained and coverslipped) with a minimum of 5,000 total nucleated cells

Collection Instructions: Submit 1-2 slides stained and coverslipped.

Additional Information: Cytology slides will not be returned.


Specimen Minimum Volume

Formalin-fixed, paraffin-embedded (FFPE) tissue block (preferred) or 1 slide stained with hematoxylin and eosin and 8 unstained, nonbaked slides

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Frozen 
  Refrigerated 

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday through Friday; Varies

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

RAS/RAF Targeted Gene Panel by Next Generation Sequencing, Tumor

81210-BRAF (v-raf murine sarcoma viarl oncogene humolog B1) (eg, colon cancer), gene analysis, V600E variant

81275-KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene) (eg, carcinoma) gene analysis, variants in codons 12 and 13

81403-HRAS (v-Ha-ras Harvey rat sarcoma viral oncogene homolog) (eg, Costello syndrome), exon 2 sequence

81404-NRAS (neuroblastoma RAS viral oncogene homolog) (eg, colorectal carcinoma), exon 1 and exon 2 sequence

Slide Review

88381-Microdissection, manual

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RASFP RAS/RAF Panel, Tumor In Process

 

Result ID Test Result Name Result LOINC Value
36725 Result Summary 50397-9
36726 Result No LOINC Needed
36727 Interpretation 69047-9
36728 Additional Information 48767-8
36729 Specimen 31208-2
36730 Source 31208-2
36731 Tissue ID No LOINC Needed
36732 Released By No LOINC Needed

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request Form (T729) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/oncology-request-form.pdf)