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Test ID VRERP Vancomycin-Resistant Enterococcus, Molecular Detection, PCR

Useful For

Identifying carriers of vancomycin-resistant enterococci

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

VRE PCR

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Vancomycin-Resistant Enterococcus DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Supplies:

Culturette (BBL Culture Swab) (T092)

C and S Vial (T058)

Stool container, Small (Random), 4 oz Random (T288)

 

Preferred:

Specimen Type: Perianal, perirectal, rectal

Container/Tube: Culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium [T092])

Specimen Volume: Swab

 

Acceptable:

Specimen Type: Preserved Stool

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair, Para-Pak C and S [T058])

Specimen Volume: Representative portion of stool

Collection Instructions:

1. Collect fresh stool and submit 1 gram or 5 mL in container with transport medium.

2. Place stool in preservative within 2 hours of collection.

 

Specimen Type: Unpreserved stool

Container/Tube: Stool container (Supply T288)

Specimen Volume: Representative portion of stool


Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Medical Laboratories in Rochester

CPT Code Information

87500

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VRERP VRE PCR In Process

 

Result ID Test Result Name Result LOINC Value
SRC60 Specimen source 31208-2
84406 VRE PCR 71718-1

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).