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Test ID ALC Ethanol, Blood

Useful For

Detection of ethanol (ethyl alcohol) in blood to document prior consumption or administration of ethanol


Quantification of the concentration of ethanol in blood correlates directly with degree of intoxication

Method Name

Headspace Gas Chromatography-Flame Ionization Detector (HSGC-FID)

Reporting Name

Ethanol, B

Specimen Type

Whole Blood NaFl-KOx

Advisory Information

This test is not performed using chain of custody. For chain of custody testing order COCH / Chain-of-Custody Processing.

Specimen Required


Preferred: Grey top (potassium oxalate/sodium fluoride)

Acceptable: Any anticoagulant

Specimen Volume: 2 mL

Collection Instructions: Specimen must be sent in original tube.

Specimen Minimum Volume

0.5 mL or amount to fill 1 tube

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood NaFl-KOx Refrigerated (preferred) 72 hours
  Frozen  14 days
  Ambient  24 hours

Reference Values

Not detected (Positive results are quantified.)

Limit of detection: 10 mg/dL (0.01 g/dL)

Legal limit of intoxication is 80 mg/dL (0.08 g/dL).

Toxic concentration is dependent upon individual usage history.

Potentially lethal concentration: ≥400 mg/dL (0.4 g/dL)

Day(s) and Time(s) Performed

Monday through Sunday; Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information


G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ALC Ethanol, B 56478-1


Result ID Test Result Name Result LOINC Value
30908 Ethanol, B 56478-1

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

NY State Approved



If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.