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Test ID ANA2 Antinuclear Antibodies (ANA), Serum

Useful For

Evaluating patients suspected of having a systemic rheumatic disease

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Antinuclear Ab, S

Specimen Type


Ordering Guidance

If suspicious of connective tissue disorder, see CTDC / Connective Tissue Disease Cascade, Serum.


If suspicious of autoimmune liver disease, see ALDG / Autoimmune Liver Disease Panel, Serum.

Specimen Required


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reference Values

≤1.0 U (negative)

1.1-2.9 U (weakly positive)

3.0-5.9 U (positive)

≥6.0 U (strongly positive)

Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANA2 Antinuclear Ab, S 94875-2


Result ID Test Result Name Result LOINC Value
ANA2 Antinuclear Ab, S 94875-2

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Testing Algorithm

See Connective Tissue Disease Cascade (CTDC) in Special Instructions.

Report Available

1 day

NY State Approved



If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Gastroenterology and Hepatology Client Test Request (T728)

-Renal Diagnostics Test Request (T830)