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Test ID ANAES Bacterial Culture, Anaerobic with Antimicrobial Susceptibilities, Varies


Shipping Instructions


Specimen should arrive within 72 hours of collection.

 

1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.



Necessary Information


Specimen source is required.



Specimen Required


Supplies:

-Anaerobic Transport Tube (T588)

-Infectious Container, Large (T146)

Acceptable Sources: Abscesses, percutaneous transtracheal aspirates, sterile body fluids, suprapubic aspirations, or wounds

Supplies: Anaerobe Transport Tube (T588)

Collection Instructions: Specimen should be obtained by using a needle and syringe from a source not normally colonized by anaerobes.


Useful For

Diagnosing anaerobic bacterial infections

 

Directing antimicrobial therapy for anaerobic infections

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ANAID Anaerobe Ident No, (Bill Only) No
RMALA Id MALDI-TOF Mass Spec Anaerobe No, (Bill Only) No
BATTA Anaerobe Suscep Battery No, (Bill Only) No
ISAN Anaerobe Ident by Sequencing No, (Bill Only) No
TISSR Tissue Processing No, (Bill Only) No
SANA Anaerobe Suscep per agent No, (Bill Only) No
BLA Beta Lactamase No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No

Testing Algorithm

When this test is ordered the reflex tests may be performed and charged separately. All bacterial organisms submitted will automatically have susceptibility testing performed and billed as appropriate. Antimicrobial agents appropriate to the organism and specimen source will be tested according to Mayo's practice and the laboratory's standard operating procedures.

 

See Anaerobic Bacteria Antimicrobials in Special Instructions to review the table that provides a listing of the antimicrobials routinely tested in our laboratory as well as antimicrobials that may be tested upon request. Call 800-533-1710 and ask to speak to the Bacteriology Anaerobe Laboratory if the organism or antimicrobial of interest is not listed in this table.

Special Instructions

Method Name

Conventional Culture Techniques with Minimum Inhibitory Concentration (MIC) by Agar Dilution (if appropriate)

Reporting Name

Bacterial Culture, Anaerobic + Susc

Specimen Type

Varies

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient 72 hours

Reference Values

No growth

 

Identification of probable pathogens

 

Results are reported as minimal inhibitory concentration (MIC) in mcg/mL. Breakpoints (also known as "clinical breakpoints") are used to categorize an organism as susceptible, susceptible-dose dependent, intermediate, resistant, or nonsusceptible according to the Clinical and Laboratory Standards Institute (CLSI) guidelines.

 

In some instances an interpretive category cannot be provided based on available data and the following comment will be included: "There are no established interpretive guidelines for agents reported without interpretations."

 

Susceptible (S):

A category defined by a breakpoint that implies that isolates with an MIC at or below the susceptible breakpoint are inhibited by the usually achievable concentrations of antimicrobial agent when the dosage recommended to treat the site of infection is used, resulting in likely clinical efficacy.

 

Intermediate (I):

A category defined by a breakpoint that includes isolates with MICs within the intermediate range that approach usually attainable blood and tissue levels and for which response rates may be lower than for susceptible isolates.

Note: The intermediate category implies clinical efficacy in body sites where the drugs are physiologically concentrated or when a higher than normal dosage of a drug can be used. This category also includes a buffer zone, which should prevent small, uncontrolled, technical factors from causing major discrepancies in interpretations, especially for drugs with narrow pharmacotoxicity margins.

 

Resistant (R):

A category defined by a breakpoint that implies that isolates with an MIC at or above the resistant breakpoint are not inhibited by the usually achievable concentrations of the agent with normal dosage schedules and/or that demonstrate MICs that fall in the range in which specific microbial resistance mechanisms are likely, and clinical efficacy of the agent against the isolate has not been reliably shown in treatment studies.

 

Epidemiological Cutoff Value (ECV):

The minimum inhibitory concentration (MIC) that separates microbial populations into those with and without acquired resistance (non-wild-type or wild-type, respectively). The ECV defines the highest MIC for the wild type population of isolates. ECVs are based on in vitro data only, using MIC distributions. ECVs are not clinical breakpoints, and the clinical relevance of ECVs for a particular patient has not yet been identified or approved by CLSI or any regulatory agency.

 

When an ECV is reported, the following comment will be included: "This MIC is consistent with the Epidemiological Cutoff Value (ECV) observed in isolates [WITH / WITHOUT] acquired resistance; however, correlation with treatment outcome is unknown."

 

(Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing. 29th edition. CLSI supplement M100. Wayne, PA, 2019)

Day(s) and Time(s) Performed

Monday through Sunday; Continuously

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87075-Bacterial Culture, Anaerobic

87076-Anaerobe Ident (if appropriate)

87076-Id MALDI-TOF Mass Spec Anaerobe (if appropriate)

87153-Anaerobe Ident by Sequencing (if appropriate)

87186-Antimicrobial Susceptibility, Anaerobic Bacteria, MIC (if appropriate)

87181-Anaerobe Susceptibility per agent (if appropriate) 

87185-Beta Lactamase (if appropriate)

87176-Tissue Processing (if appropriate)

87798-Identification by PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANAES Bacterial Culture, Anaerobic + Susc 635-3

 

Result ID Test Result Name Result LOINC Value
ANAES Bacterial Culture, Anaerobic + Susc 635-3

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.