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HelpTest ID BAKDM BCR/ABL1, Tyrosine Kinase Inhibitor Resistance, Kinase Domain Mutation Screen, Sanger Sequencing, Varies
Useful For
Evaluating patients with chronic myelogenous leukemia and Philadelphia chromosome positive B-cell acute lymphoblastic leukemia receiving tyrosine kinase inhibitor (TKI) therapy, who are apparently failing treatment
Preferred initial test to identify the presence of acquired BCR-ABL1 mutations associated with TKI-resistance
Special Instructions
Method Name
Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with Sanger Sequencing
Reporting Name
BCR/ABL1 Mutation, SequencingSpecimen Type
VariesOrdering Guidance
This is the preferred initial test to identify the presence of acquired BCR/ABL1 mutations associated with tyrosine kinase inhibitor (TKI)-resistance.
Shipping Instructions
1. Refrigerated specimens must arrive within 5 days of collection, and ambient specimens must arrive within 3 days (72 hours) of collection.
2. Draw and package specimen as close to shipping time as possible.
Necessary Information
The following information is required:
1. Patient's fusion type (p210, p190, p205 or p230)
2. Pertinent clinical history
3. Clinical or morphologic suspicion
4. Date of collection
5. Specimen source (blood or bone marrow)
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 10 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Acceptable:
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Minimum Volume
Blood: 8 mL
Bone Marrow: 2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 5 days | PURPLE OR PINK TOP/EDTA |
| Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81170-ABL1 (ABL proto-ongogene 1, non-receptor tyrosine kinase)(eg, acquired imatinib tyrosine kinase inhibitor resistance), gene analysis, variants in the kinase domain
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BAKDM | BCR/ABL1 Mutation, Sequencing | 55135-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP004 | Specimen Type | 31208-2 |
| MOFF | BCRABL Fusion (210, 190, 205, 230) | 55135-8 |
| 19824 | Final Diagnosis: | 34574-4 |
Test Classification
This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.Testing Algorithm
If BCR/ABL1 fusion type (p210, p190, p205 or p230) is not provided, BADX / BCR/ABL1, Qualitative, Diagnostic Assay, Varies will be performed at an additional charge.
In the event that no fusion form (p190, p205, p210, p230) is identified by qualitative testing, this test will be canceled.
For more information see BCR/ABL1 Ordering Guide for Blood and Bone Marrow.
Report Available
5 to 7 daysNY State Approved
YesForms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BADX | BCR/ABL1, RNA-Qual, Diagnostic | Yes | No |