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HelpTest ID BLPF B-Cell Lymphoma, FISH, Varies
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various B-cell lymphomas using blood or bone marrow specimens
Tracking known chromosome abnormalities and response to therapy in patients with B-cell neoplasms
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _PBCT | Probe, +2 | No, (Bill Only) | No |
| _PADD | Probe, +1 | No, (Bill Only) | No |
| _PB02 | Probe, +2 | No, (Bill Only) | No |
| _PB03 | Probe, +3 | No, (Bill Only) | No |
| _IL25 | Interphases, <25 | No, (Bill Only) | No |
| _I099 | Interphases, 25-99 | No, (Bill Only) | No |
| _I300 | Interphases, >=100 | No, (Bill Only) | No |
Testing Algorithm
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
When this test and flow cytometry testing for leukemia/lymphoma are ordered concurrently, the flow cytometry result will be utilized to determine if sufficient clonal B-cells are available for FISH testing. If the result does not identify a sufficient clonal B-cell population, this FISH test order will be canceled and no charges will be incurred.
If FISH testing proceeds, probes will be performed based on the lymphoma subtype suspected/identified utilizing the table "Common Chromosome Abnormalities in B-cell Lymphomas" located in Clinical Information.
If the patient is being tracked for known abnormalities, indicate which probes should be used.
The following probe sets are available within this B-cell lymphoma FISH profile:
-8q24.1 rearrangement, MYC
-t(8;14), MYC/IGH
-18q21 rearrangement, BCL2
-3q27 rearrangement, BCL6
-t(11;14), CCND1/IGH
-17p deletion, TP53/D17Z1
-7q deletion, D7Z1/7q32
-18q21 rearrangement, MALT1
Marginal zone lymphoma (MZL) can be evaluated via probes for specific subtypes, as follows: mucosa-associated lymphoid tissue (MALT) lymphoma or extra-nodal marginal zone lymphoma (ENMZL) using the MALT1 rearrangement probe and splenic marginal zone lymphoma using probes for 7q deletion and 17p deletion. If no MZL subtype is provided, we will evaluate using probes for MALT1, 7q deletion and 17p deletion.
See Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm in Special Instructions.
Special Instructions
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
B-cell Lymphoma, FISH, B/BMSpecimen Type
VariesAdvisory Information
This assay detects chromosome abnormalities observed in the blood or bone marrow of patients with B-cell lymphoma. If a formalin-fixed, paraffin-embedded specimen is submitted, the test will be cancelled and BLYM / B-Cell Lymphoma, FISH, Tissue will be added and performed as the appropriate test.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
1. Provide a reason for testing with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
2. A pathology or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Bone marrow
Container/Tube: Green top (sodium heparin)
Specimen Volume: 1-2 mL
Collection Instructions: Invert several times to mix bone marrow.
Acceptable:
Specimen Type: Blood
Container/Tube: Green top (sodium heparin)
Specimen Volume: 7-10 mL
Collection Instructions: Invert several times to mix blood.
Specimen Type: Touch prep or fresh tissue
Specimen Minimum Volume
Blood: 2 mL
Bone Marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Specimens are processed Monday through Sunday.
Results reported Monday through Friday; 8 a.m. to 5 p.m.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
88271x2, 88291-DNA probe, each (first probe set), Interpretation and report
88271x2-DNA probe, each; each additional probe set (if appropriate)
88271x1-DNA probe, each; coverage for sets containing 3 probes (if appropriate)
88271x2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)
88271x3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)
88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)
88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)
88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BLPF | B-cell Lymphoma, FISH, B/BM | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 51900 | Result Summary | 50397-9 |
| 51902 | Interpretation | 69965-2 |
| 51901 | Result Table | 93356-4 |
| 54546 | Result | 62356-1 |
| CG684 | Reason for Referral | 42349-1 |
| CG685 | Specimen | 31208-2 |
| 51903 | Source | 31208-2 |
| 51904 | Method | 49549-9 |
| 54455 | Additional Information | 48767-8 |
| 53867 | Disclaimer | 62364-5 |
| 51905 | Released by | 18771-6 |
NY State Approved
YesForms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.