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HelpTest ID BTWGS Bacterial Typing, Whole Genome Sequencing, Varies
Infectious
Useful For
Aiding in the investigation of a potential outbreak by a single bacterial species
May assist in identification of recurrent infection in an individual patient
Special Instructions
Method Name
Whole Genome Sequencing
Reporting Name
Bacterial Typing, Whole Genome SeqSpecimen Type
VariesOrdering Guidance
Acinetobacter baumannii, Campylobacter jejuni/coli, Clostridioides difficile (formerly Clostridium difficile), Cutibacterium (Propionibacterium) acnes, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus lugdunensis, Streptococcus agalactiae, and Streptococcus pyogenes are tested by this method.
Additional Testing Requirements
If uncertain of organism identification, concomitantly order IDENT / Organism Referred for Identification, Aerobic Bacteria or ANIDE / Organism Referred for Identification, Anaerobic Bacteria.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place isolates in a large infectious container and label as an etiologic agent/infectious substance.
3. Place all isolates (all patients and/or sites to be compared) submitted for whole genome sequencing together in 1 large bag and send in the same shipping container. This is necessary for comparison of isolates by this method.
Necessary Information
1. Organism identification and specimen source are required.
2. Acinetobacter baumannii: Isolate must be A baumannii. Identifications of A baumannii complex and A baumannii/calcoaceticus complex are not acceptable.
3. Enterobacter cloacae: Isolate must be a member of the E cloacae complex (ie, Enterobacter asburiae, Enterobacter bugandensis, E cloacae, E cloacae subspecies cloacae, E cloacae subspecies dissolvens, Enterobacter hormaechei subspecies hormaechei, Enterobacter kobei, Enterobacter ludwigii, Enterobacter xiangfangensis).
4. Klebsiella pneumoniae: Isolate must be a member of the K pneumoniae complex (ie, K pneumoniae, K pneumoniae subspecies pneumoniae, K pneumoniae subspecies ozaenae, or K pneumoniae subspecies rhinoscleromatis).
5. An email address registered on the Mayo Clinic Laboratories website is required for report delivery.
Specimen Required
Aerobic Bacteria
Supplies: Infectious Container, Large (T146)
Container/Tube: Agar slant
Specimen Volume: Isolates on separate agar slants in pure culture
Collection Instructions:
1. Isolate the bacteria (must be Acinetobacter baumannii, Campylobacter jejuni/coli, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus lugdunensis, Streptococcus agalactiae, or Streptococcus pyogenes).
2. Bacterial isolate must be in pure culture, actively growing. Do not submit mixed cultures.
3. Each isolate must be submitted under a separate order.
Anaerobic Bacteria
Supplies:
-Anaerobe Transport Tube (T588)
-Infectious Container, Large (T146)
Container/Tube:
Preferred: Anaerobic transport tube
Acceptable: Thioglycollate broth or any other suitable anaerobic transport system
Specimen Volume: Isolates in separate transport tubes in pure culture
Collection Instructions:
1. Isolate the bacteria (must be Clostridioides difficile or Cutibacterium [Propionibacterium] acnes).
2. Do not submit growth directly from a CHROMagar plate; subculture to anaerobic media to obtain pure isolate and confirm identification prior to submission.
3. Bacterial isolate must be in pure culture, actively growing. Do not submit mixed cultures.
4. Each isolate must be submitted under a separate order.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
Reported as isolates are "related," "possibly related," or "unrelated."
Day(s) Performed
Once per week
Report Available
30 to 40 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
0010U-Bacterial Typing, Whole Genome Seq
87900-Bioinformatics Reanalysis (if appropriate)
87077-Ident by MALDI-TOF mass spec (if appropriate)
87153-Aerobe ident by sequencing (if appropriate)
87077-Additional identification procedure (if appropriate)
87076-Id MALDI-TOF mass spec anaerobe (if appropriate)
87076-Anaerobe Ident (if appropriate)
87153-Anaerobe ident by sequencing (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BTWGS | Bacterial Typing, Whole Genome Seq | 90246-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BTWGS | Bacterial Typing, Whole Genome Seq | 90246-0 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| BIORE | Bioinformatics Reanalysis | No, (Bill Only) | No |
| RMALD | Ident by MALDI-TOF mass spec | No, (Bill Only) | No |
| ISAE | Aerobe Ident by Sequencing | No, (Bill Only) | No |
| REFID | Additional Identification Procedure | No, (Bill Only) | No |
| RMALA | Id MALDI-TOF Mass Spec Anaerobe | No, (Bill Only) | No |
| ANAID | Anaerobe Ident | No, (Bill Only) | No |
| ISAN | Anaerobe Ident by Sequencing | No, (Bill Only) | No |
Testing Algorithm
Organism identification is required. All bacteria submitted will be tested. Organism identifications that are incorrect or incomplete may result in no results at final analysis. Laboratory may perform matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) or other testing as listed in Reflex Tests to confirm identification and report this finding at an additional charge.
When reanalysis of previously submitted isolates for comparison to new isolates is requested, bioinformatics reanalysis will be added by Mayo Clinic Laboratories. Original patient submission information (names and order numbers) must be provided.
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.