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Test ID CDGF Celiac Disease Gluten-Free Cascade, Serum and Whole Blood

Useful For

Evaluating patients suspected of having celiac disease who are currently (or were recently) on a gluten-free diet

Method Name

CELI2: Polymerase Chain Reaction (PCR)/Sequence-Specific Oligonucleotide Probe (SSO)

Reporting Name

Celiac Disease Gluten-Free Cascade

Specimen Type

Whole Blood ACD-B

Advisory Information

Cascade testing is recommended for celiac disease. Cascade testing ensures that testing proceeds in an algorithmic fashion. The following cascades are available; select the appropriate one for your specific patient situation.

-CDCOM / Celiac Disease Comprehensive Cascade: complete testing including HLA DQ

-CDSP / Celiac Disease Serology Cascade: complete testing excluding HLA DQ

-CDGF / Celiac Disease Gluten-Free Cascade: for patients already adhering to a gluten-free diet

To order individual tests, see Celiac Disease Diagnostic Testing Algorithm in Special Instructions.

Specimen Required

Both blood and serum are required.


Specimen Type: Blood

Container/Tube: Yellow top (ACD solution B)

Specimen Volume: 6 mL

Collection Instructions: Do not transfer blood to other containers.


Specimen Type: Serum


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 2 mL

Specimen Minimum Volume

Blood: 3 mL
Serum 1.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 21 days
  Frozen  21 days
Whole Blood ACD-B Refrigerated (preferred)

Reference Values


Presence of DQ2 or DQ8 alleles associated with celiac disease

Day(s) and Time(s) Performed

IgA, tTG IgA, Gliadin IgA, Gliadin IgG, tTG IgG: Monday through Saturday; 3 p.m.

HLA-DQ Typing: Monday through Friday; 7:30 a.m.-5 p.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81376 x 2

82784-IgA (if appropriate)

83516-Deamidated gliadin IgA (if appropriate)

83516-Deamidated gliadin IgG (if appropriate)

83516-tTG IgA (if appropriate)

83516-tTG IgG (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CDGF Celiac Disease Gluten-Free Cascade 94493-4


Result ID Test Result Name Result LOINC Value
DQA DQ alpha 1 94495-9
28991 Celiac Disease Interpretation 69048-7
DQB DQ beta 1 53938-7
CELIG Celiac gene pairs present? 48767-8

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Profile Information

Test ID Reporting Name Available Separately Always Performed
CELI2 HLA-DQ Typing Yes, (Order CELI) Yes
CDGF1 Celiac Disease Interpretation No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
TTGA Tissue Transglutaminase Ab, IgA, S Yes No
DAGL Gliadin(Deamidated) Ab, IgA, S Yes No
DGGL Gliadin(Deamidated) Ab, IgG, S Yes No
TTGG Tissue Transglutaminase Ab, IgG, S Yes No
IGA Immunoglobulin A (IgA), S Yes No

Testing Algorithm

If HLA-DQ typing is positive or equivocal for DQ2 or DQ8, then IgA, tissue transglutaminase (tTG) IgA and IgG, and deamidated gliadin IgA and IgG will be performed at an additional charge.


The following algorithms are available in Special Instructions:

-Celiac Disease Comprehensive Cascade

-Celiac Disease Diagnostic Testing Algorithm

-Celiac Disease Gluten-Free Cascade

-Celiac Disease Routine Treatment Monitoring Algorithm

-Celiac Disease Serology Cascade

NY State Approved



If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.