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HelpTest ID CDGF Celiac Disease Gluten-Free Cascade, Serum and Whole Blood
Useful For
Evaluating patients suspected of having celiac disease who are currently (or were recently) on a gluten-free diet
Special Instructions
Method Name
CELI2: Polymerase Chain Reaction (PCR)/Sequence-Specific Oligonucleotide Probe (SSO)
Reporting Name
Celiac Disease Gluten-Free CascadeSpecimen Type
SerumWhole Blood ACD-B
Advisory Information
-CDCOM / Celiac Disease Comprehensive Cascade: complete testing including HLA DQ
-CDSP / Celiac Disease Serology Cascade: complete testing excluding HLA DQ
-CDGF / Celiac Disease Gluten-Free Cascade: for patients already adhering to a gluten-free diet
To order individual tests, see Celiac Disease Diagnostic Testing Algorithm in Special Instructions.
Specimen Required
Both blood and serum are required.
Specimen Type: Blood
Container/Tube: Yellow top (ACD solution B)
Specimen Volume: 6 mL
Collection Instructions: Do not transfer blood to other containers.
Specimen Type: Serum
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Volume: 2 mL
Specimen Minimum Volume
Blood: 3 mL
Serum 1.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days | ||
| Whole Blood ACD-B | Refrigerated (preferred) | ||
| Ambient | |||
Reference Values
HLA-DQ TYPING
Presence of DQ2 or DQ8 alleles associated with celiac disease
Day(s) and Time(s) Performed
IgA, tTG IgA, Gliadin IgA, Gliadin IgG, tTG IgG: Monday through Saturday; 3 p.m.
HLA-DQ Typing: Monday through Friday; 7:30 a.m.-5 p.m.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81376 x 2
82784-IgA (if appropriate)
83516-Deamidated gliadin IgA (if appropriate)
83516-Deamidated gliadin IgG (if appropriate)
83516-tTG IgA (if appropriate)
83516-tTG IgG (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CDGF | Celiac Disease Gluten-Free Cascade | 94493-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| DQA | DQ alpha 1 | 94495-9 |
| 28991 | Celiac Disease Interpretation | 69048-7 |
| DQB | DQ beta 1 | 53938-7 |
| CELIG | Celiac gene pairs present? | 48767-8 |
Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CELI2 | HLA-DQ Typing | Yes, (Order CELI) | Yes |
| CDGF1 | Celiac Disease Interpretation | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TTGA | Tissue Transglutaminase Ab, IgA, S | Yes | No |
| DAGL | Gliadin(Deamidated) Ab, IgA, S | Yes | No |
| DGGL | Gliadin(Deamidated) Ab, IgG, S | Yes | No |
| TTGG | Tissue Transglutaminase Ab, IgG, S | Yes | No |
| IGA | Immunoglobulin A (IgA), S | Yes | No |
Testing Algorithm
If HLA-DQ typing is positive or equivocal for DQ2 or DQ8, then IgA, tissue transglutaminase (tTG) IgA and IgG, and deamidated gliadin IgA and IgG will be performed at an additional charge.
The following algorithms are available in Special Instructions:
-Celiac Disease Comprehensive Cascade
-Celiac Disease Diagnostic Testing Algorithm
-Celiac Disease Gluten-Free Cascade
NY State Approved
YesForms
If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.