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Test ID CEE20 CD20 Cell Expression Evaluation, Varies


Advisory Information


This test should not be used as a shortened diagnostic panel. For a complete diagnostic B-cell, T-cell, or acute immunophenotyping panel, order LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies.

 

This test evaluates CD20 expression only. For CD52 expression, order CEE52 / CD52 Cell Expression Evaluation, Varies. For CD49d expression, order CEE49 / CD49d Cell Expression Evaluation, Varies.



Shipping Instructions


It is recommended that specimens arrive within 24 hours of draw. Draw and package specimen as close to shipping time as possible.



Necessary Information


The following information is required:

1. The therapeutic monoclonal antibody being used or considered

2. The pertinent hematologic diseases that have been diagnosed or considered

3. Specimen source

4. Date and time of collection



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: Sodium heparin or EDTA

Specimen Volume: 10 mL

Collection Instructions:

1. Do not transfer blood to other containers.

2. Label specimen as blood.

Specimen Stability Information: Ambient/Refrigerated

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: Sodium heparin or EDTA

Specimen Volume: 1-5 mL

Collection Instructions:

1. Label specimen as bone marrow.

2. Submission of bilateral specimens is not required.

Specimen Stability Information: Ambient/Refrigerated


Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Useful For

Detecting cell-surface antigens on malignant cells that are potential therapeutic antibody targets, specifically CD20

 

Determining the eligibility of patients for monoclonal antibody therapies

 

Monitoring response to the therapeutic antibody

Testing Algorithm

A complete diagnostic B-cell, T-cell, or acute immunophenotyping panel is not performed. In some cases, a limited morphologic evaluation will be performed.

Method Name

Immunophenotyping

Reporting Name

CD20 Cell Expression Evaluation, V

Specimen Type

Varies

Specimen Minimum Volume

Blood: 3 mL
Bone Marrow Aspirate: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Varies 4 days

Reference Values

Normal individuals have B lymphocytes, T lymphocytes, or myeloid cells that express the corresponding cell-surface antigens in question.

Day(s) and Time(s) Performed

Specimens are processed Monday through Sunday.

Results reported Monday through Friday.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker

88185 x 3-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker

88187-Flow Cytometry Interpretation, 2 to 8 Markers

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CEE20 CD20 Cell Expression Evaluation, V In Process

 

Result ID Test Result Name Result LOINC Value
CK164 CEE20 Result No LOINC Needed
CK165 Final Diagnosis 22637-3

NY State Approved

Yes