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Test ID E157C Escherichia coli O157:H7 Culture, Feces

Additional Testing Requirements

In some cases, local public health requirements may impact Mayo Clinic Laboratories clients, requiring, for example, submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Escherichia coli O157:H7 species recovered from specimens submitted to Mayo Clinic Laboratories by calling 800-533-1710 as soon as possible after reporting (to ensure viability of the bacterium).

Shipping Instructions

Specimen must arrive within 96 hours of collection.

Necessary Information

Specimen source is required.

Specimen Required

Patient Preparation: Medications: Do not use barium or bismuth before collection of specimen.

Supplies: C and S Vial (T058)

Specimen Type: Preserved Feces

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.

Useful For

Determining whether Escherichia coli O157:H7 may be the cause of diarrhea


Reflexive testing for Shiga toxin and/or E coli O157:H7 nucleic acid amplification test-positive feces


This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.


This test provides evidence of the presence of the bacterium, Escherichia coli O157:H7, in feces, in a viable state, and provides an isolate for submission to a health department, if needed. Minnesota healthcare providers are required to report all confirmed or suspected cases of E coli O157:H7 and other Shiga toxin-producing E coli to the Minnesota Department of Health. Mayo Clinic Laboratories clients should refer to their local health departments regarding public health submission of E coli O157:H7 and other Shiga toxin-producing E coli isolates.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
EC Serologic Agglut Method 2 Ident No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged separately.


See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Method Name

Conventional Culture

Reporting Name

E. coli O157:H7 Culture, F

Specimen Type


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 4 days
  Refrigerated  4 days

Reference Values

No growth of pathogen

Day(s) Performed

Monday through Sunday 

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87046-Escherichia coli O157:H7 Culture, Stool-with isolation and preliminary examination

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87147-Serologic Agglut Method 2 Ident (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
E157C E. coli O157:H7 Culture, F 10851-4


Result ID Test Result Name Result LOINC Value
E157C E. coli O157:H7 Culture, F 10851-4

NY State Approved