Test ID ERPR Estrogen/Progesterone Receptor, Semi-Quantitative Immunohistochemistry, Manual
Ordering Guidance
This test is for prognostic purposes only.
For diagnostic purposes, order PATHC / Pathology Consultation and then request the stains.
Estrogen/progesterone receptor testing is not appropriate and not performed for cases of lobular carcinoma in situ.
Shipping Instructions
Attach the green pathology address label included in the kit to the outside of the transport container.
Necessary Information
1. Include accompanying pathology report stating the final diagnosis. If not available, a preliminary diagnosis is acceptable.
2. Information regarding fixative used, time to fixation, and duration of fixation is required. The following questions, as stated on the order form or presented electronically, must be answered:
a. "Was specimen fixed in 10% neutral buffered formalin within 1 hour from surgical collection time? Yes, No, or Unknown."
b. "Has specimen been fixed in 10% neutral buffered formalin for 6 to 72 hours? Yes, No, or Unknown."
c. "Was tissue decalcified? Yes, No, or Unknown."
d. "Tumor type? Primary breast carcinoma, metastatic breast carcinoma, or non-breast tumor."
e. "Tumor classification? Invasive breast carcinoma, ductal carcinoma in situ, metastatic breast carcinoma, micro-invasive breast carcinoma, solid/intracystic papillary carcinoma, or non-breast tumor."
Specimen Required
Supplies: Pathology Packaging Kit (T554)
Specimen Type: Breast carcinoma
Preferred: A paraffin-embedded tissue block containing in-situ, invasive or metastatic breast carcinoma tissue that has been fixed in 10% neutral buffered formalin within 1 hour from surgical collection time and for a total of 6 to 72 hours and shipped at ambient temperature
Acceptable: 3 unstained sections, containing carcinoma, on charged slides cut at 4 microns <1 month ago and shipped at ambient temperature
Collection Instructions: Submit paraffin-embedded carcinoma tissue
Specimen Type: Non-breast carcinoma
Preferred: A paraffin-embedded tissue block containing carcinoma tissue that has been fixed in 10% neutral buffered formalin and shipped at ambient temperature
Acceptable: 3 unstained sections, containing carcinoma, on charged slides cut at 4 microns <1 month ago and shipped at ambient temperature
Collection Instructions: Submit paraffin-embedded carcinoma tissue
Additional Information:
1. According to the American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines, estrogen/progesterone receptor protein immunohistochemical test results are only valid for nondecalcified, paraffin-embedded specimens fixed in 10% neutral buffered formalin within 1 hour from surgical collection time and for a total time of 6 to 72 hours. Delay to fixation, under- or overfixation may affect these results.
2. Paraffin blocks will be returned with final report.
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Oncology Test Request (T729)
-Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763)
Useful For
Guiding decisions on hormonal therapy in patients with breast carcinomas
This test is not useful for cases of lobular carcinoma in situ.
Method Name
Semi-Quantitative Immunohistochemistry
Reporting Name
ER/PR Semi Quant IHC ManualSpecimen Type
SpecialSpecimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Special | Ambient (preferred) | ||
Refrigerated |
Reference Values
Negative: <1% reactive cells
Positive: ≥1% reactive cells
Day(s) Performed
Monday through Friday
Report Available
4 to 6 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
88360 x 2
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ERPR | ER/PR Semi Quant IHC Manual | 10480-2 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
70965 | Interpretation | 50595-8 |
70966 | Participated in the Interpretation | No LOINC Needed |
70967 | Report electronically signed by | 19139-5 |
70969 | Material Received | 81178-6 |
MA002 | Fixed in 10% NB formalin w/in 1 hr | 8100-0 |
MA003 | Fixed in 10% NB formalin 6-72 hrs | 8100-0 |
MA004 | Tumor type | 44638-5 |
MA005 | Tumor classification | 21918-8 |
MA044 | Tissue was decalcified | 8100-0 |
71620 | Disclaimer | 62364-5 |
71834 | Case Number | 80398-1 |