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Test ID ESTS Estradiol, Rapid, Immunoassay, Serum

Useful For

Rapid assessment of ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)


Establishing time of ovulation and optimal time for conception

Method Name

Electrochemiluminescent Immunoassay

Reporting Name

Estradiol Rapid, Immunoassay, S

Specimen Type


Ordering Guidance

This assay is for reproductive assessment (eg, IVF, conception). For other clinical indications, order EEST / Estradiol, Serum.


The preferred method for measurement of low serum estradiol concentrations in children, males, and postmenopausal females is liquid chromatography-tandem mass spectrometry (LC-MS/MS), order EEST / Estradiol, Serum.

Specimen Required

Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen (preferred) 180 days
  Refrigerated  5 days
  Ambient  24 hours

Reference Values

Males: 10-40 pg/mL


Premenopausal: 15-350 pg/mL*

Postmenopausal: <10 pg/mL

*Estradiol concentrations vary widely throughout the menstrual cycle

The limit of quantitation for estradiol measured by immunoassay is 25 pg/mL. Mass spectrometry is the preferred method for measurement of low serum estradiol concentrations in children, males and postmenopausal females (EEST / Estradiol, Serum).

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
ESTS Estradiol Rapid, Immunoassay, S 2243-4


Result ID Test Result Name Result LOINC Value
ESTS Estradiol Rapid, Immunoassay, S 2243-4

NY State Approved