Test ID F13NG F13A1 and F13B Genes, Next-Generation Sequencing, Varies
Advisory Information
Genetic testing should only be considered if reduced factor XIII activity is documented and acquired cases of low factor XIII are excluded.
Shipping Instructions
Ambient and refrigerated specimens must arrive within 7 days (168 hours of draw), and frozen specimens must arrive within 14 days (336 hours of draw).
Collect and package specimen as close to shipping time as possible.
Necessary Information
Rare Coagulation Disorder Patient Information is required, see Special Instructions. Testing may proceed without the patient information, however, the information aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Peripheral blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD) or green top (sodium citrate)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
Specimen Stability: Ambient (preferred)/Refrigerated/Frozen
Specimen Type: Extracted DNA
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. Label specimen as extracted DNA and source of specimen.
2. Provide volume and concentration of the DNA.
Specimen Stability: Frozen (preferred)/Refrigerated/Ambient
Forms
1. Rare Coagulation Disorder Patient Information (T824) is required, see Special Instructions. Fax the completed form to 507-284-1759.
2. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:
-Informed Consent for Genetic Testing (T576)
-Informed Consent for Genetic Testing-Spanish (T826)
3. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.
Useful For
Detecting the pathogenic alterations within the F13A1 and F13B genes to delineate the underlying molecular defect in a patient with a laboratory diagnosis of factor XIII deficiency
Genetic confirmation of hereditary factor XIII deficiency with the identification of an alteration in either the F13A1 or F13B gene known or suspected to cause the condition
Testing for close family members of an individual with a factor XIII deficiency diagnosis
This test is not useful for prenatal diagnosis
Genetics Test Information
The gene targets for this test are:
Gene name (transcript): F13A1 (GRCh37 [hg19] NM_000129)
Chromosomal location: 6p24-p25
Gene name (transcript): F13B (GRCh37 [hg19] NM_001994)
Chromosomal location: 1q31-q32.1
Testing Algorithm
A standard testing algorithm for factor 13 deficiency (FXIIID) has been developed by the Scientific and Standardization Committee of the International Society for Thrombosis and Haemostasis (ISTH).(1)
Genetic testing for factor XIII deficiency is indicated if:
-Factor XIII activity (FXIII) is reduced on a qualitative functional FXIII activity test
-Acquired causes of factor XIII deficiency have been excluded
Special Instructions
Method Name
Custom Sequence Capture and Targeted Next-Generation Sequencing (NGS) Followed by Polymerase Chain Reaction (PCR) and Sanger Sequencing When Appropriate
Reporting Name
F13A1 and B Genes, Full Gene NGSSpecimen Type
VariesSpecimen Minimum Volume
Blood: 1 mL
Extracted DNA: 100 mcL at 50 ng/mcL
concentration
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Ambient (preferred) | 7 days | |
Frozen | 14 days | ||
Refrigerated | 7 days |
Reference Values
An interpretive report will be provided
Day(s) and Time(s) Performed
Performed weekly, Varies
Performing Laboratory

Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
81479
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
F13NG | F13A1 and B Genes, Full Gene NGS | 92991-9 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
113076 | F13NG Result | 50397-9 |
113070 | Alterations Detected | 82939-0 |
113069 | Interpretation | 69047-9 |
113071 | Additional Information | 48767-8 |
113072 | Method | 85069-3 |
113073 | Disclaimer | 62364-5 |
113074 | Panel Gene List | 48018-6 |
113075 | Reviewed By | 18771-6 |