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Test ID F5M 5-Methyltetrahydrofolate

Method Name


Reporting Name


Specimen Type


Specimen Required

Medical Neurogenetics collection kit (T657) required.

Each collection kit contains 5 microcentrifuge tubes.



1)     CSF should be collected from the first drop into the tubes in the numbered order. Fill each tube to the marked line with the required volumes

Tube 1: 0.5 mL

Tube 2: 1.0 mL

Tube 3: 1.0 mL (contains antioxidants necessary to protect the sample integrity)

Tube 4: 1.0 mL

Tube 5: 1.0 mL

-       If sample's not blood contaminated, the tubes should be placed on dry ice at bedside

-       If sample's are blood contaminated, the tubes should immediately be centrifuged (prior to freezing) and the clear CSF transferred to new similarly labeled tubes, then frozen

-       Store samples at -80 until they can be shipped

2)     Complete Medical Neurogenetics, LLC request form. Include test required, sample date and date of birth.  

3)     Label tubes with patient name and ID number, leaving the tube number viewable.

4)     Place samples inside a specimen transport bag and the Medical Neurogenetics, LLC request form inside the pouch of the transport bag.

5)     Ship samples frozen on dry ice.

Specimen Stability Information

Specimen Type Temperature Time Special Container

Reference Values


Age           5MTHF             

(years)      (nmol/L)           


0-0.2         40-240

0.2-0.5      40-240

0.5-2.0      40-187

2.0-5.0      40-150   

5.0-10       40-128  

10-15        40-120   

Adults       40-120



Note: If test results are inconsistent with the clinical presentation, please call our laboratory to discuss the case and/or submit a second sample for confirmatory testing.


DISCLAIMER required by the FDA for high complexity clinical laboratories: HPLC testing was developed and its performance characteristics determined by Medical Neurogenetics. These HPLC tests have not been cleared or approved by the U.S. FDA.

Day(s) Performed

Monday through Friday

Performing Laboratory

Medical Neurogenetics, LLC

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
F5M 5-Methyltetrahydrofolate 47547-5


Result ID Test Result Name Result LOINC Value
Z2241 5-Methyltetrahydrofolate 47547-5
Z2242 Interpretation Not Provided

Report Available

10 to 18 days

NY State Approved