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Test ID GLIOF 1p/19q Deletion in Gliomas, FISH, Tissue

Useful For

Aids in diagnosing oligodendroglioma tumors and predicting the response of an oligodendroglioma to therapy


May be useful in tumors with a complex "hybrid" morphology requiring differentiation from pure astrocytomas to support the presence of oligodendroglial differentiation/lineage


Indicated when a diagnosis of oligodendroglioma, both low-grade World Health Organization (WHO, grade II) and anaplastic (WHO, grade III) is rendered


Strongly recommended when a diagnosis of mixed oligoastrocytomas is rendered

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_I099 Interphases, 25-99 No, (Bill Only) No
_I300 Interphases, >=100 No, (Bill Only) No
_IL25 Interphases, <25 No, (Bill Only) No
_PADD Probe, +1 No, (Bill Only) No
_PB02 Probe, +2 No, (Bill Only) No
_PB03 Probe, +3 No, (Bill Only) No
_PBCT Probe, +2 No, (Bill Only) No

Testing Algorithm

This test does not include a pathology consult. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate fluorescence in situ hybridization (FISH) test will be ordered and performed at an additional charge.


This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.


Chromosomal microarray (CMAPT / Chromosomal Microarray, Tumor, Formalin-Fixed Paraffin-Embedded), rather than FISH, may be of benefit to evaluate for acquired alterations associated with the molecular classification of glioma.(1) See Cytogenetic Analysis of Glioma.

Special Instructions

Method Name

Fluorescence In Situ Hybridization (FISH) Using DNA Probes

Reporting Name

1p/19q Deletion, Glioma, FISH, Ts

Specimen Type


Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

A reason for testing and pathology report are required in order for testing to be performed. Send information with specimen. Acceptable pathology reports include working drafts, preliminary pathology, or surgical pathology reports.

Specimen Required

Submit only 1 of the following specimens:


Specimen Type: Tissue

Preferred: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.


Acceptable: Slides

Collection Instructions: Six consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

Specimen Minimum Volume

Four consecutive, unstained, 5-micron-thick sections placed on positively charged slides and 1 hematoxylin and eosin-stained slide

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Ambient (preferred)

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Report Available

8 to 12 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88271x2, 88291- DNA probe, each (first probe set), Interpretation and report

88271x2- DNA probe, each; each additional probe set (if appropriate)

88271x1- DNA probe, each; coverage for sets containing 3 probes (if appropriate)

88271x2- DNA probe, each; coverage for sets containing 4 probes (if appropriate)

88271x3- DNA probe, each; coverage for sets containing 5 probes (if appropriate)

88274- w/modifier 52- Interphase in situ hybridization, <25 cells, each probe set (if appropriate)

88274- Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GLIOF 1p/19q Deletion, Glioma, FISH, Ts In Process


Result ID Test Result Name Result LOINC Value
52107 Result Summary 50397-9
52109 Interpretation 69965-2
52108 Result 62356-1
CG739 Reason For Referral 42349-1
52110 Specimen 31208-2
52111 Source 31208-2
52112 Tissue ID 80398-1
52113 Method 85069-3
54579 Additional Information 48767-8
53836 Disclaimer 62364-5
52114 Released By 18771-6

NY State Approved