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HelpTest ID HBRP Histoplasma capsulatum/Blastomyces species, Molecular Detection, PCR, Varies
Useful For
Rapid detection of Histoplasma capsulatum and Blastomyces dermatitidis DNA
An aid in the rapid diagnosis of histoplasmosis and blastomycosis
Special Instructions
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
Histoplasma/Blastomyces PCRSpecimen Type
VariesAdvisory Information
Urine is not an acceptable source for this assay. Studies indicate that Histoplasma DNA is not routinely found in the urine of patients with disseminated histoplasmosis. Therefore, the UHIST / Histoplasma Antigen, Urine is the recommended test for this specimen source.
Additional Testing Requirements
This test should always be performed in conjunction with fungal culture, order FGEN / Fungal Culture, Routine.
Shipping Instructions
Specimen must arrive within 7 days of collection; specimens received after 7 days will be rejected.
NALC/NaOH-digested specimen must arrive within 7 days of digestion.
Necessary Information
Specimen source is required.
Specimen Required
The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Histoplasma or Blastomyces species DNA is not likely.
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Body, CSF, bone marrow
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Respiratory
Sources: BAL, bronchial washing, sputum
Container/Tube: Sterile container
Specimen Volume: 1 mL
Specimen Type: Tissue or bone
Container/Tube: Sterile container
Specimen Volume: 5-10 mm
Collection Instructions: Collect a fresh tissue or bone specimen.
Acceptable:
Specimen Type: NALC/NaOH-digested respiratory specimens
Sources: Lavage fluid, bronchial washing, gastric washing, respiratory fluid, sputum, or tracheal secretion
Container/Tube: Sterile container
Specimen Volume: 2 mL
Collection Instructions:
1. Submit digested specimen treated with NALC/NaOH.
2. Clearly indicate on container and order form that specimen is a digested specimen.
Specimen Minimum Volume
Body Fluid or Respiratory Specimen: 0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Ambient | 7 days | ||
| Frozen | 7 days |
Reference Values
Not applicable
Day(s) and Time(s) Performed
Monday through Friday, 3 times per week
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HBRP | Histoplasma/Blastomyces PCR | 81653-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| SRC78 | Histo/Blasto Source | 31208-2 |
| 32457 | Histo/Blasto Result | 81653-8 |
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.NY State Approved
YesForms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Testing Algorithm
See Meningitis/Encephalitis Panel Algorithm in Special Instructions.