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Test ID HBRP Histoplasma capsulatum/Blastomyces species, Molecular Detection, PCR, Varies

Useful For

Rapid detection of Histoplasma capsulatum and Blastomyces dermatitidis DNA

 

An aid in the rapid diagnosis of histoplasmosis and blastomycosis

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Histoplasma/Blastomyces PCR

Specimen Type

Varies


Advisory Information


Urine is not an acceptable source for this assay. Studies indicate that Histoplasma DNA is not routinely found in the urine of patients with disseminated histoplasmosis. Therefore, the UHIST / Histoplasma Antigen, Urine is the recommended test for this specimen source.



Additional Testing Requirements


This test should always be performed in conjunction with fungal culture, order FGEN / Fungal Culture, Routine.



Shipping Instructions


Specimen must arrive within 7 days of collection; specimens received after 7 days will be rejected.

 

NALC/NaOH-digested specimen must arrive within 7 days of digestion.



Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Histoplasma or Blastomyces species DNA is not likely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: Body, CSF, bone marrow

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Respiratory

Sources: BAL, bronchial washing, sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Tissue or bone

Container/Tube: Sterile container

Specimen Volume: 5-10 mm

Collection Instructions: Collect a fresh tissue or bone specimen.

 

Acceptable:

Specimen Type: NALC/NaOH-digested respiratory specimens

Sources: Lavage fluid, bronchial washing, gastric washing, respiratory fluid, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions:

1. Submit digested specimen treated with NALC/NaOH.

2. Clearly indicate on container and order form that specimen is a digested specimen.


Specimen Minimum Volume

Body Fluid or Respiratory Specimen: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday, 3 times per week

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HBRP Histoplasma/Blastomyces PCR 81653-8

 

Result ID Test Result Name Result LOINC Value
SRC78 Histo/Blasto Source 31208-2
32457 Histo/Blasto Result 81653-8

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Testing Algorithm

See Meningitis/Encephalitis Panel Algorithm in Special Instructions.

NY State Approved

Yes