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Test ID HIQNP HIV-1 RNA Detection and Quantification, Prenatal, Plasma


Ordering Guidance


For detection and quantification of HIV-1 RNA in plasma before and during antiretroviral therapy in pregnant individuals only. For nonpregnant individuals, order HIVQN / HIV-1 RNA Detection and Quantification, Plasma.



Shipping Instructions


Ship specimen frozen on dry ice. If shipment will be delayed for more than 24 hours, freeze plasma specimen at -20 to -80° C until shipment



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Centrifuge blood collection tube and aliquot plasma into plastic vial per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).

2. Freeze aliquoted plasma for shipment.


Useful For

Diagnosis of HIV-1 infection in pregnant individuals with acute or early HIV-1 infection

 

Quantifying plasma HIV-1 RNA levels (viral load) in pregnant individuals living with HIV-1:

-Before initiating anti-HIV-1 drug therapy (baseline viral load)

-Who may have developed HIV-1 drug resistance while on anti-HIV-1 therapy

-Who may be noncompliant with anti-HIV-1 drug therapy

 

Monitoring HIV-1 disease progression before or during antiretroviral drug therapy in pregnant individuals

Method Name

Real-Time Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Reporting Name

HIV-1 RNA Detect/Quant Prenatal, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.8 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 84 days
  Refrigerated  6 days

Reference Values

Undetected

Day(s) Performed

Monday through Saturday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87536

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HIQNP HIV-1 RNA Detect/Quant Prenatal, P 70241-5

 

Result ID Test Result Name Result LOINC Value
65567 HIV-1 RNA Detect/Quant Prenatal, P 70241-5

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.