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HelpTest ID HIVP HIV-1 DNA and RNA Qualitative Detection by PCR, Plasma
Useful For
Virologic detection of HIV-1 infection in infants younger than 2 years of age (an age group for which serologic tests are unreliable) born to HIV-1-infected mothers
Early detection of acute HIV-1 infection in children and adults who may be receiving combination antiretroviral prophylaxis or preemptive treatment
Determining eradication of HIV-1 in individuals receiving combination highly active antiretroviral therapies
Testing Algorithm
The following algorithms are available in Special Instructions:
-HIV Prenatal Testing Algorithm, Including Follow-up of Reactive Rapid Serologic Test Results
Special Instructions
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
HIV-1 DNA / RNA Qualitative, PSpecimen Type
Plasma EDTAAdvisory Information
This assay should not be used as a screening test or primary diagnostic test for HIV-1 infection, except in infants younger than 2 years of age born to HIV-1-infected mothers. For other age groups, order HVCOP / HIV-1 and HIV-2 Antigen and Antibody Routine Screen, Plasma as the screening test.
Shipping Instructions
1. Ship plasma on ice packs if arrival at Mayo Clinic Laboratories is expected within 5 days of plasma separation.
2. If shipment will be delayed for more than 5 days, freeze plasma specimen -20 to -80° CÂ until shipment on dry ice.
Specimen Required
Supplies: Aliquot Tube, 5 mL (T465)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge blood collection tube and aliquot plasma into plastic vial per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
Additional Information: This test can be used for detection and diagnosis of HIV-1 infections, including in children younger than 2 years of age when serologic tests are not useful (due to presence of maternal HIV antibodies).
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen (preferred) | 35 days | |
| Refrigerated | 5 days |
Reference Values
Undetected
Day(s) and Time(s) Performed
Varies (once per week minimum)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
87535
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HIVP | HIV-1 DNA / RNA Qualitative, P | 79379-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 64693 | HIV-1 DNA / RNA Qualitative, P | 79379-4 |