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HelpTest ID HIVP HIV-1 DNA and RNA Qualitative Detection by PCR, Plasma
Useful For
Virologic detection of HIV-1 infection in infants younger than 2 years of age (an age group for which serologic tests are unreliable) born to HIV-1-infected mothers
Early detection of acute HIV-1 infection in children and adults who may be receiving combination antiretroviral prophylaxis or preemptive treatment
Determining eradication of HIV-1 in individuals receiving combination highly active antiretroviral therapies
Testing Algorithm
The following algorithms are available in Special Instructions:
-HIV Prenatal Testing Algorithm, Including Follow-up of Reactive Rapid Serologic Test Results
Special Instructions
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
HIV-1 DNA / RNA Qualitative, PSpecimen Type
Plasma EDTAOrdering Guidance
This assay should not be used as a screening test or primary diagnostic test for HIV-1 infection, except in infants younger than 2 years of age born to HIV-1-infected mothers. For other age groups, order HVCOP / HIV-1 and HIV-2 Antigen and Antibody Routine Screen, Plasma as the screening test.
Shipping Instructions
1. Ship plasma on ice packs if arrival at Mayo Clinic Laboratories is expected within 5 days of plasma separation.
2. If shipment will be delayed for more than 5 days, freeze plasma specimen -20 to -80° CÂ until shipment on dry ice.
Specimen Required
Supplies: Aliquot Tube, 5 mL (T465)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge blood collection tube and aliquot plasma into plastic vial per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
Additional Information: This test can be used for detection and diagnosis of HIV-1 infections, including in children younger than 2 years of age when serologic tests are not useful (due to presence of maternal HIV antibodies).
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen (preferred) | 35 days | |
| Refrigerated | 5 days |
Reference Values
Undetected
Day(s) Performed
Varies
Report Available
3 to 10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
87535
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HIVP | HIV-1 DNA / RNA Qualitative, P | 79379-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 64693 | HIV-1 DNA / RNA Qualitative, P | 79379-4 |