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Test ID HIVPR HIV-1 Genotypic Protease and Reverse Transcriptase Inhibitor Drug Resistance, Plasma

Useful For

Identification of HIV-1 genotypic mutations associated with resistance to nucleotide reverse-transcriptase inhibitors, non-nucleotide reverse-transcriptase inhibitors, and protease inhibitors


Guiding initiation or change of drug combinations for the treatment of HIV-1 infection

Testing Algorithm

See HIV Treatment Monitoring Algorithm in Special Instructions.

Special Instructions

Method Name

Reverse Transcription-Polymerase Chain Reaction (RT-PCR)/DNA Sequencing

Reporting Name

HIV-1 Genotypic PR-RT Resistance, P

Specimen Type

Plasma EDTA

Specimen Required

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.2 mL

Collection Instructions:

1. Spin down and remove plasma from cells within 6 hours of draw.

2. Freeze plasma specimen immediately, and ship specimen frozen on dry ice.

3. If shipment will be delayed for >24 hours, freeze plasma specimen at -70° C (up to 35 days) until shipment on dry ice.

Additional Information:

1. This test is intended to be used to monitor known HIV-positive infections. It is not intended for primary detection of HIV infections.

2. Specimens submitted for HIV-1 genotyping should contain ≥500 copies/mL of HIV-1 RNA.

Specimen Minimum Volume

1.2 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen (preferred) 35 days
  Refrigerated  24 hours

Reference Values

Not applicable

Day(s) and Time(s) Performed

Varies; test will be performed in batches of 10

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
HIVPR HIV-1 Genotypic PR-RT Resistance, P 49659-6


Result ID Test Result Name Result LOINC Value
37216 HIV-1 Genotypic PR-RT Drug Resistance, P 34700-5
37263 Nucleos(t)ide RT mutations 45175-7
21411 Abacavir 30287-7
21406 Didanosine 30284-4
37285 Emtricitabine 41402-9
37284 Lamivudine 30283-6
21408 Stavudine 30286-9
21530 Tenofovir 41396-3
21405 Zidovudine 30282-8
37264 Nonnucleoside RT mutations 45176-5
21414 Efavirenz 30291-9
31267 Etravirine 52749-9
21410 Nevirapine 30289-3
34917 Rilpivirine 68463-9
21400 Protease Mutations 33630-5
28076 Atazanavir + Ritonavir 49618-2
26784 Darunavir + Ritonavir 49630-7
26733 Fosamprenavir + Ritonavir 51409-1
26734 Indinavir + Ritonavir 49619-0
21532 Lopinavir + Ritonavir 42000-0
21416 Nelfinavir 30294-3
26735 Saquinavir + Ritonavir 49621-6
28201 Tipranavir + Ritonavir 49622-4