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Test ID HVA Homovanillic Acid, 24 Hour, Urine

Useful For

Screening children for catecholamine-secreting tumors with a 24-hour urine collection when requesting homovanillic acid only

 

Monitoring neuroblastoma treatment

 

Screening patients with possible inborn errors of catecholamine metabolism

Highlights

Homovanillic acid (HVA) measurement in urine is used for screening children for catecholamine-secreting tumors such as neuroblastoma, pheochromocytoma, and other neural crest tumors and monitoring those who have had treatment for these tumors.

 

HVA measurement is also useful to diagnose children with disorders of catecholamine metabolism such as monoamine oxidase-A deficiency and dopamine beta-hydroxylase deficiency, which results in either decreased or elevated urinary HVA values, respectively.

 

Treatment with L-dopa can impact test results and should be discontinued 24 hours prior to collection. Bactrim can impact test results and should be noted at time of collection.

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Homovanillic Acid (HVA), 24 Hr, U

Specimen Type

Urine


Necessary Information


1. Collection duration and urine volume are required.

2. Patient's age is required.



Specimen Required


Patient Preparation: Administration of L-dopa may falsely increase homovanillic acid results; it should be discontinued 24 hours prior to and during collection of specimen.

Container/Tube: Plastic, 10-mL urine tube (T068)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a 24-hour urine specimen.

2. Add 25 mL of 50% acetic acid as preservative at start of collection. If specimen is refrigerated during collection, preservative may be added up to 12 hours after collection. Use 15 mL of 50% acetic acid for children less than 5 years old. This preservative is intended to achieve a pH of between approximately 1 and 5. If necessary, adjust urine pH to 1 to 5 with 50% acetic or HCI acid.

Additional Information:

1. The sensitivity of this test is greater on a 24-hour specimen than on a random specimen.

2. See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens in Special Instructions for multiple collections.

3. Bactrim may interfere with detection of the analyte. All patients taking Bactrim should be identified to the laboratory when this test is ordered.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 28 days
  Frozen  180 days

Reference Values

<1 year: <35.0 mg/g creatinine

1 year: <30.0 mg/g creatinine

2-4 years: <25.0 mg/g creatinine

5-9 years: <15.0 mg/g creatinine

10-14 years: <9.0 mg/g creatinine

≥15 years (adults): <8 mg/24 hours

Day(s) and Time(s) Performed

Monday through Friday; 8 a.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83150

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HVA Homovanillic Acid (HVA), 24 Hr, U 13760-4

 

Result ID Test Result Name Result LOINC Value
3572 Homovanillic Acid, Adult (>14 yr) 2436-4
3573 Homovanillic Acid, Child (<15 yr) 13760-4
TM39 Collection Duration 13362-9
VL37 Urine Volume 3167-4

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

NY State Approved

Yes

Urine Preservative Collection Options

Note: The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection.

Ambient

No

Refrigerate

No

Frozen

No

50% Acetic Acid

Preferred

Boric Acid

No

Diazolidinyl Urea

No

6M Hydrochloric Acid

Yes

6M Nitric Acid

No

Sodium Carbonate

No

Thymol

No

Toluene

No