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Test ID IABCS B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood

Important Note

Two separate EDTA specimens are required: 1 refrigerated and 1 at ambient transport temperature.  Specimens to be collected Monday-Wednesday ONLY. For serial monitoring, we recommend that specimen draws be performed at the same time of day.

Useful For

Screening for common variable immunodeficiency and hyper-IgM syndromes

 

Assessing B-cell subset reconstitution after stem cell or bone marrow transplant

 

Assessing response to B-cell-depleting immunotherapy

 

This test is not indicated for the evaluation of lymphoproliferative disorders (eg, leukemia, lymphoma, multiple myeloma).

Method Name

Flow Cytometry

Reporting Name

Immune Assessment B Cell Subsets, B

Specimen Type

Whole Blood EDTA


Ordering Guidance


This test requires multiple whole blood specimens to perform all testing. If only one whole blood specimen type is received, only the testing associated with that specimen type will be performed. Be advised that this may change the degree of interpretation received with the report. If only the refrigerate EDTA sample is received, this test will be canceled and converted to RBCS / Relative B-Cell Subset Analysis Percentage, Blood, which provides the relative B-cell subset values without quantitation.

 

This test is a screening test, and further analyses will be required to complete a diagnostic workup for common variable immunodeficiency (CVID) (eg, CVID / Common Variable Immunodeficiency Confirmation Flow Panel, Blood) and hyper-IgM (XHIM / X-Linked Hyper IgM Syndrome, Blood and CD40 / B-Cell CD40 Expression by Flow Cytometry, Blood).



Shipping Instructions


Specimens must be received in the laboratory weekdays and by 4 p.m. on Friday. Collect and package specimens as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of collection.

 

Samples arriving on the weekend and observed holidays may be canceled.



Necessary Information


1. Date of collection is required.

2. Ordering physician's name and phone number are required.



Specimen Required


Two separate EDTA whole blood specimens are required: 1 refrigerated and 1 at ambient transport temperature.

 

For serial monitoring, it is recommended that specimens are collected at the same time of day.

 

Specimen Type: Whole blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer (NK) Cells, Blood

Container/Tube: 4 mL Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions:

1. Send whole blood specimen in original tube. Do not aliquot.

2. Label specimen as blood for TBBS

Specimen Stability Information: Ambient <52 hours

 

Specimen Type: Whole blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 10 mL

Pediatric (≤14 years old) Volume: 4 mL

Collection Instructions:

1. Send whole blood specimen in original tube. Do not aliquot.

2. Label specimen as blood for IABC.

Specimen Stability Information: Refrigerated <48 hours


Specimen Minimum Volume

TBBS: 1 mL
IABC: 5 mL; for pediatric patients: 3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Varies 48 hours PURPLE OR PINK TOP/EDTA

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86355-B cells, total count

86357-Natural killer (NK) cells, total count

86359-T cells, total count

86360-Absolute CD4/CD8 count with ratio

86356 x7 - Mononuclear cell antigen, quantitative

LOINC Code Information

Test ID Test Order Name Order LOINC Value
IABCS Immune Assessment B Cell Subsets, B 90416-9

 

Result ID Test Result Name Result LOINC Value
30296 CD19+ % of total Lymphocytes 8117-4
3321 CD45 Total Lymph Count 27071-0
3316 % CD3 (T Cells) 8124-0
29094 CD20+ % of total Lymphocytes 8119-0
3322 CD3 (T Cells) 8122-4
30298 CD27+ % of CD19+ B cells 89358-6
3319 % CD4 (T Cells) 8123-2
30300 CD27+ IgM+ IgD+ % of CD19+ B cells 89352-9
3325 CD4 (T Cells) 24467-3
30302 CD27+ IgM- IgD- % of CD19+ B cells 89350-3
3320 % CD8 (T Cells) 8101-8
30304 CD27+ IgM+ IgD- % of CD19+ B cells 89348-7
3326 CD8 (T Cells) 14135-8
30306 IgM+ % of CD19+ B cells 89346-1
3318 % CD19 (B Cells) 8117-4
30308 CD38+ IgM- % of CD19+ B cells 89344-6
30310 CD38+ IgM+ % of CD19+ B cells 89341-2
3324 CD19 (B Cells) 8116-6
4054 % CD16+CD56 (NK cells) 8112-5
30312 CD21+ % of CD19+ B cells 89356-0
30314 CD21- % of CD19+ B cells 89355-2
4055 CD16+CD56 (NK cells) 20402-4
3327 4/8 Ratio 54218-3
30297 CD19+ 8116-6
29095 CD20+ 9558-8
6657 Comment 80722-2
30299 CD27+ 89353-7
30301 CD27+ IgM+ IgD+ 89351-1
30303 CD27+ IgM- IgD- 89349-5
30305 CD27+ IgM+ IgD- 89347-9
30307 IgM+ 89345-3
30309 CD38+ IgM- 89343-8
30311 CD38+ IgM+ 89357-8
30313 CD21+ 25164-5
30315 CD21- 89354-5
30316 Interpretation 80722-2

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Profile Information

Test ID Reporting Name Available Separately Always Performed
TBBS QN Lymphocyte Subsets: T, B, and NK Yes Yes
IABC Immune Assessment B Cell Subsets, B No Yes

Testing Algorithm

When multiple specimen types are required to perform a panel of tests, the laboratory will perform the tests for which the appropriate specimen type was received. The laboratory will cancel those for which the appropriate specimen was not received. Be advised that this may change the degree of interpretation received with the report.

Report Available

3 to 4 days

NY State Approved

Yes

Disease States

  • Common variable immunodeficiency