Test ID IETG Interference Evaluation Heterophile, Thyroglobulin Tumor Marker, Serum

Ordering Guidance

If interference or heterophile testing is not required, order HTG2 / Thyroglobulin, Tumor Marker, Serum.

For fine-needle aspirate specimens, order TFNAB / Thyroglobulin, Tumor Marker, Fine-Needle Aspiration Biopsy Needle Wash.

Specimen Required

Patient Preparation: For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: None (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Useful For

Evaluation of suspected interference from heterophile antibodies causing a falsely elevated thyroglobulin result

Profile Information

Test ID	Reporting Name	Available Separately	Always Performed
TGII	TG, Interference Interpretation	No	Yes
TGQN	Thyroglobulin, Tumor Marker, S	Yes, (Order HTG2)	Yes
TGABI	Thyroglobulin Antibody, S	Yes, (Order HTG2)	Yes
TGMS	Thyroglobulin, Mass Spec., S	Yes	Yes

Testing Algorithm

Heterophile antibody evaluation consists of comparison of thyroglobulin (Tg) concentrations obtained by immunoassay with the following:

-Tg concentrations following pretreatment with commercial heterophile blocking reagents

-Tg concentrations obtained by mass spectrometry

-Tg concentrations obtained by serial dilutions of the sample

In all samples the presence of anti-thyroglobulin antibodies are evaluated.

Method Name

TGII: Medical Interpretation

TGQN/TGABI: Immunoenzymatic Assay

TGMS: Tryptic Protein Fragmentation, purified with Immunocapture, Analysis by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

(This service is performed pursuant to an agreement with SISCAPA Assay Technologies Inc. covering US Patent 7,632,686)

Reporting Name

Interference Eval, Heterophile, TG

Specimen Type

Serum Red

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type	Temperature	Time
Serum Red	Refrigerated (preferred)	7 days
	Frozen	30 days
	Ambient	72 hours

Reference Values

THYROGLOBULIN TUMOR MARKER

≤33 ng/mL

THYROGLOBULIN, MASS SPECTROMETRY

≤33 ng/mL

THYROGLOBULIN ANTIBODY

<1.8 IU/mL

Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

Report Available

3 to 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84432

86800