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Test ID KNSRP KPC (blaKPC) and NDM (blaNDM) Surveillance, PCR, Varies

Useful For

Identifying carriers of carbapenem-resistant Enterobactericeae harboring KPC (Klebsiella pneumoniae carbapenemase) or NDM (New Dehli metallo-beta-lactamase) genes

Method Name

Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

KPC and NDM Surveillance PCR

Specimen Type

Varies


Advisory Information


This assay should be used for surveillance testing on perirectal/rectal swabs or fecal specimens. If testing isolates from culture, order KPNRP / KPC (blaKPC) and NDM (blaNDM) in Gram-Negative Bacilli, Molecular Detection, PCR, Varies.



Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by KPC or NDM DNA is not likely.

 

Submit only 1 of the following specimens:

 

Supplies:

-Culturette (BBL Culture Swab) (T092)

-C and S Vial (T058)

 

Preferred:

Specimen Type: Perianal, perirectal, rectal

Collection Container/Tube: Culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)

Specimen Volume: Swab

 

Acceptable:

Specimen Type: Preserved feces

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of feces


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
KNSRP KPC and NDM Surveillance PCR 85502-3

 

Result ID Test Result Name Result LOINC Value
SRCKP Specimen source 31208-2
35165 KPC PCR 85499-2
35166 NDM PCR 85500-7

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.