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Test ID LCMSP Microsporidia species, Molecular Detection, PCR, Varies

Useful For

Detection of Enterocytozoon bieneusi and Encephalitozoon species in fecal and urine specimens to support the clinical diagnosis of microsporidiosis

Highlights

This test is intended to be used for immunocompromised patients with persistent, watery diarrhea. See the Parasitic Investigation of Stool Specimens Algorithm.

 

This test is intended to be used for the detection of Enterocytozoon bieneusi and Encephalitozoon species DNA in fecal and urine specimens to support the clinical diagnosis of microsporidiosis. Other sources should be tested using MTBS / Microsporidia Stain, Varies.

 

A negative result indicates the absence of detectable DNA from Enterocytozoon bieneusi and Encephalitozoon species in the specimen, but does not rule out ongoing microsporidiosis. See Interpretation for more information.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Microsporidia PCR

Specimen Type

Varies


Ordering Guidance


For specimens other than feces or urine, MTBS / Microsporidia Stain, Varies should be ordered since it will detect other microsporidia that are not targeted by this assay.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Unpreserved feces

Supplies: Stool container, Small (Random), 4 oz (T288); Stool Collection Kit, Random (T635)

Container/Tube: Fecal container (T288)

Specimen Volume: 5 g

 

Specimen Type: Preserved feces

Supplies: ECOFIX Stool Transport Vial (Kit) (T219); Stool Collection Kit, Random (T635)

Container/Tube: ECOFIX preservative

Specimen Volume: 5 g

 

Specimen Type: Urine

Container/Tube: Sterile container

Specimen Volume: 5 mL

Collection Instructions: Mid-stream, clean-catch, suprapubic aspirates and catheterization collections are acceptable. Please submit in a clean, sterile container free from preservatives. The first portion of the voided urine (first void) is also acceptable.


Specimen Minimum Volume

Feces: 1 g
Urine: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reference Values

Negative

Day(s) Performed

Tuesday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LCMSP Microsporidia PCR 94332-4

 

Result ID Test Result Name Result LOINC Value
MSPS Specimen Source 31208-2
36744 Encephalitozoon species 94333-2
36745 Enterocytozoon bieneusi 94334-0

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Testing Algorithm

The following algorithms are available in Special Instructions:

-Parasitic Investigation of Stool Specimens Algorithm

-Laboratory Testing for Infectious Causes of Diarrhea

Report Available

2 to 4 days

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Gastroenterology and Hepatology Client Test Request (T728)