Test ID LPCBS Lysophosphatidylcholines by LC MS/MS, Blood Spot
Useful For
Second-tier newborn screen for X-linked adrenoleukodystrophy (X-ALD)
Genetics Test Information
This test is used as a second-tier newborn screen for X-linked adrenoleukodystrophy (X-ALD).
Highlights
Testing for C24:0 lysophosphatidylcholines (LPC) and C26:0 LPC can aid in the diagnosis of X-linked adrenoleukodystrophy (X-ALD) as well as other types of peroxisomal disorders.
Analysis of LPCs in a blood spot test is a useful second-tier newborn screen test for X-ALD.
Special Instructions
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
LysoPC by LC MS/MS, BSSpecimen Type
Whole bloodSpecimen Required
Supplies: Card-Blood Spot Collection (Filter Paper) (T493)
Container/Tube:
Preferred: Blood Spot Collection Card (T493)
Acceptable: Ahlstrom 226 filter paper, Munktell and Whatman Protein Saver 903 Paper
Specimen Volume: 2 blood spots
Collection Instructions:
1. Completely fill at least 2 circles on the filter paper card (approximately 100 microliters blood per circle).
2. Let blood dry on the filter paper at ambient temperature in a horizontal position for a minimum of 3 hours.
3. Do not expose specimen to heat or direct sunlight.
4. Do not stack wet specimens.
5. Keep specimen dry.
Additional Information:
1. For collection instructions in Spanish, see Blood Spot Collection Card-Spanish Instructions (T777) in Special Instructions.
2. For collection instructions in Chinese, see Blood Spot Collection Card-Chinese Instructions (T800) in Special Instructions.
Specimen Minimum Volume
Blood spot: 1
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole blood | Refrigerated (preferred) | 56 days | FILTER PAPER |
Frozen | 56 days | FILTER PAPER | |
Ambient | 7 days | FILTER PAPER |
Reference Values
Analyte |
Normal Range (mcg/mL) |
C20 Lysophosphatidylcholine |
Not applicable |
C22 Lysophosphatidylcholine |
Not applicable |
C24 Lysophosphatidylcholine |
≤0.25 |
C26 Lysophosphatidylcholine |
≤0.20 |
Day(s) and Time(s) Performed
Varies
Performing Laboratory

CPT Code Information
82542
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
LPCBS | LysoPC by LC MS/MS, BS | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
34865 | Interpretation (LPCBS) | 59462-2 |
34860 | C20 Lysophosphatidylcholine | 90920-0 |
34861 | C22 Lysophosphatidylcholine | 90921-8 |
34862 | C24 Lysophosphatidylcholine | 90922-6 |
34863 | C26 Lysophosphatidylcholine | 90923-4 |
34864 | Reviewed By | 18771-6 |
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.NY State Approved
YesForms
1. Biochemical Genetics Patient Information (T602) in Special Instructions
2. If not ordering electronically, complete, print, and send an Inborn Errors of Metabolism Test Request (T798) with the specimen.
Testing Algorithm
For more information, see the following documents in Special Instructions:
-Newborn Screening Act Sheet X-linked Adrenoleukodystrophy: Increased Very Long Chain Fatty Acids
-Newborn Screen Follow-up for X-linked Adrenoleukodystrophy