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HelpTest ID MFCF Myeloma, FISH, Fixed Cells
Useful For
Aiding in the diagnosis of new cases of multiple myeloma
Identifying prognostic markers based on the abnormalities found
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _PBCT | Probe, +2 | No, (Bill Only) | No |
| _PADD | Probe, +1 | No, (Bill Only) | No |
| _PB02 | Probe, +2 | No, (Bill Only) | No |
| _PB03 | Probe, +3 | No, (Bill Only) | No |
| _IL25 | Interphases, <25 | No, (Bill Only) | No |
| _I099 | Interphases, 25-99 | No, (Bill Only) | No |
| _I300 | Interphases, >=100 | No, (Bill Only) | No |
Testing Algorithm
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
This test is designed for diagnostic specimens.
Indicate if the entire panel is to be performed. If the patient is being tracked for known abnormalities, indicate which probes should be used.
Panel includes testing for the following abnormalities using the probes listed:
-13/13q-, RB1/LAMP1
t(11;14), CCND1/IGH
14q32 rearrangement, IGH
17p-, TP53/D17Z1
+3/+7, D3Z1/D7Z1
+9/+15, D9Z1/D15Z4
1q gain, TP73/1q22
8q24,1 rearrangement, MYC
When an IGH rearrangement is identified, reflex testing is performed to identify the translocation partner. Probes include identification of t(4;14)(p16.3;q32) FGFR3/IGH, t(14;16)(q32;q23) IGH/MAF, t(14;20)(q32;q12), IGH/MAFB or t(6;14)(p21;q32) CCND3/IGH.
If bone marrow is submitted, this test will be cancelled and PCPDF / Plasma Cell Proliferative Disorder (PCPD), FISH, Bone Marrow will be performed as the appropriate test.
If a formalin-fixed, paraffin-embedded specimen is submitted, this test will be cancelled and PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue will be performed as the appropriate test.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
Myeloma, FISH, Fixed CellsSpecimen Type
Fixed Cell Pellet Bone MarrowAdvisory Information
If testing is requested using formalin-fixed, paraffin-embedded specimens, order PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue.
If testing is requested using fresh bone marrow specimens, order PCPDF / Plasma Cell Proliferative Disorder (PCPD), FISH, Bone Marrow.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
1. Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
2. Provide a pathology report with each specimen.
3. A pathology and/or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.
Specimen Required
Container/Tube: Sterile container
Specimen Volume: 1 fixed cell pellet
Collection Instructions: Place specimen in a sterile container with a 3:1 (or similar) fixative (methanol:glacial acetic acid).
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fixed Cell Pellet Bone Marrow | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
88271x2, 88291-DNA probe, each (first probe set), Interpretation and report
88271x2-DNA probe, each; each additional probe set (if appropriate)
88271-DNA probe, each; coverage for sets containing 3 probes (if appropriate)
88271x2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)
88271x3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)
88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)
88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)
88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MFCF | Myeloma, FISH, Fixed Cells | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 51817 | Result Summary | 50397-9 |
| 51819 | Interpretation | 69965-2 |
| 51818 | Result Table | 36908-2 |
| 54533 | Result | 62356-1 |
| CG661 | Reason for Referral | 42349-1 |
| 51820 | Specimen | 31208-2 |
| 51821 | Source | 31208-2 |
| 51822 | Method | 49549-9 |
| 53431 | Additional Information | 48767-8 |
| 55277 | Disclaimer | 62364-5 |
| 51823 | Released By | 18771-6 |
NY State Approved
YesForms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.