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HelpTest ID MFCF Myeloma, FISH, Fixed Cells
Useful For
Aiding in the diagnosis of new cases of multiple myeloma or other plasma cell proliferative disorders
Identifying prognostic markers based on the abnormalities found
This test should not be used to track the progression of disease.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _PBCT | Probe, +2 | No, (Bill Only) | No |
| _PADD | Probe, +1 | No, (Bill Only) | No |
| _PB02 | Probe, +2 | No, (Bill Only) | No |
| _PB03 | Probe, +3 | No, (Bill Only) | No |
| _IL25 | Interphases, <25 | No, (Bill Only) | No |
| _I099 | Interphases, 25-99 | No, (Bill Only) | No |
| _I300 | Interphases, >=100 | No, (Bill Only) | No |
Testing Algorithm
This test is designed for diagnostic specimens from patients with multiple myeloma or other plasma cell proliferative disorders.
This test includes a charge for application of the first probe set (2 fluorescence in situ hybridization: FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
For diagnostic samples, all probes in the initial panel will be evaluated. The initial panel includes testing for the following abnormalities using the probes listed:
17p-, TP53/D17Z1
1q gain, TP73/1q22
14q32 rearrangement, IGH
t(11;14), CCND1/IGH
8q24.1 rearrangement, MYC
-13/13q-, RB1/LAMP1
+9/+15, D9Z1/D15Z4
+3/+7, D3Z1/D7Z1
Based on the results from the initial panel, reflex testing may be performed to identify the following abnormalities using the probes listed:
t(14;16)(q32;q23) IGH/MAF
t(4;14)(p16.3;q32) FGFR3/IGH
t(14;20)(q32;q12) IGH/MAFB
t(6;14)(p21;q32) CCND3/IGH
For follow-up samples, only TP73/1q22, TP53/D17Z1 and MYC probes, along with a single probe that was abnormal in a previous study, will be tested. If a previous sample was uninformative due to an insufficient number of plasma cells, analysis will begin with the initial panel.
If the standard algorithm is not desired, please indicate which probes should be used.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
Myeloma, FISH, Fixed CellsSpecimen Type
Fixed Cell Pellet Bone MarrowAdvisory Information
-For fresh bone marrow specimens, order PCPDS / Plasma Cell Proliferative Disorder, FISH, Bone Marrow.
-For paraffin-embedded tissue specimens, order PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue.
-Testing will be changed to the appropriate test if this test is ordered on either of the previous specimens or if bone marrow specimens are received greater than 96 hours from collection.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
A pathology and/or flow cytometry report may be requested by the laboratory if a reason for referral is not provided with the specimen.
Specimen Required
Container/Tube: Sterile container
Specimen Volume: 1 fixed cell pellet
Collection Instructions: Place specimen in a sterile container with a 3:1 methanol:glacial acetic acid (or similar) fixative.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fixed Cell Pellet Bone Marrow | Ambient (preferred) | ||
| Refrigerated | |||
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Samples processed Monday through Sunday.
Results reported Monday through Friday, 8 a.m.-5 p.m.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
88271x2, 88291-DNA probe, each (first probe set), Interpretation and report
88271x2-DNA probe, each; each additional probe set (if appropriate)
88271-DNA probe, each; coverage for sets containing 3 probes (if appropriate)
88271x2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)
88271x3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)
88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)
88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)
88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MFCF | Myeloma, FISH, Fixed Cells | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 51817 | Result Summary | 50397-9 |
| 51819 | Interpretation | 69965-2 |
| 51818 | Result Table | 93356-4 |
| 54533 | Result | 62356-1 |
| CG661 | Reason for Referral | 42349-1 |
| 51820 | Specimen | 31208-2 |
| 51821 | Source | 31208-2 |
| 51822 | Method | 49549-9 |
| 53431 | Additional Information | 48767-8 |
| 55277 | Disclaimer | 62364-5 |
| 51823 | Released By | 18771-6 |
NY State Approved
YesForms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.