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Test ID MFCF Myeloma, FISH, Fixed Cells

Useful For

Aiding in the diagnosis of new cases of multiple myeloma

 

Identifying prognostic markers based on the abnormalities found

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_PBCT Probe, +2 No, (Bill Only) No
_PADD Probe, +1 No, (Bill Only) No
_PB02 Probe, +2 No, (Bill Only) No
_PB03 Probe, +3 No, (Bill Only) No
_IL25 Interphases, <25 No, (Bill Only) No
_I099 Interphases, 25-99 No, (Bill Only) No
_I300 Interphases, >=100 No, (Bill Only) No

Testing Algorithm

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

 

This test is designed for diagnostic specimens.

 

Indicate if the entire panel is to be performed. If the patient is being tracked for known abnormalities, indicate which probes should be used.

 

Panel includes testing for the following abnormalities using the probes listed:

-13/13q-, RB1/LAMP1

t(11;14), CCND1/IGH

14q32 rearrangement, IGH

17p-, TP53/D17Z1

+3/+7, D3Z1/D7Z1

+9/+15, D9Z1/D15Z4

1q gain, TP73/1q22

8q24,1 rearrangement, MYC

 

When an IGH rearrangement is identified, reflex testing is performed to identify the translocation partner. Probes include identification of t(4;14)(p16.3;q32) FGFR3/IGH, t(14;16)(q32;q23) IGH/MAF, t(14;20)(q32;q12), IGH/MAFB or t(6;14)(p21;q32) CCND3/IGH.

 

If bone marrow is submitted, this test will be cancelled and PCPDF / Plasma Cell Proliferative Disorder (PCPD), FISH, Bone Marrow will be performed as the appropriate test.

 

If a formalin-fixed, paraffin-embedded specimen is submitted, this test will be cancelled and PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue will be performed as the appropriate test.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

Myeloma, FISH, Fixed Cells

Specimen Type

Fixed Cell Pellet Bone Marrow


Advisory Information


If testing is requested using formalin-fixed, paraffin-embedded specimens, order PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue.

 

If testing is requested using fresh bone marrow specimens, order PCPDF / Plasma Cell Proliferative Disorder (PCPD), FISH, Bone Marrow.



Shipping Instructions


Advise Express Mail or equivalent if not on courier service.



Necessary Information


1. Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

2. Provide a pathology report with each specimen.

3. A pathology and/or flow cytometry report may be requested by the Genomics Laboratory to optimize testing and aid in interpretation of results.



Specimen Required


Container/Tube: Sterile container

Specimen Volume: 1 fixed cell pellet

Collection Instructions: Place specimen in a sterile container with a 3:1 (or similar) fixative (methanol:glacial acetic acid).


Specimen Stability Information

Specimen Type Temperature Time Special Container
Fixed Cell Pellet Bone Marrow Ambient (preferred)
  Refrigerated 

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88271x2, 88291-DNA probe, each (first probe set), Interpretation and report

88271x2-DNA probe, each; each additional probe set (if appropriate)

88271-DNA probe, each; coverage for sets containing 3 probes (if appropriate)

88271x2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)

88271x3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)

88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)

88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)

88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MFCF Myeloma, FISH, Fixed Cells In Process

 

Result ID Test Result Name Result LOINC Value
51817 Result Summary 50397-9
51819 Interpretation 69965-2
51818 Result Table 36908-2
54533 Result 62356-1
CG661 Reason for Referral 42349-1
51820 Specimen 31208-2
51821 Source 31208-2
51822 Method 49549-9
53431 Additional Information 48767-8
55277 Disclaimer 62364-5
51823 Released By 18771-6

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.