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Test ID MGRP Mycoplasma genitalium, Molecular Detection, PCR, Varies

Useful For

Rapid, sensitive, and specific identification of Mycoplasma genitalium from genitourinary and reproductive sources

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

Mycoplasma genitalium PCR

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Mycoplasma genitalium DNA is not likely.

 

Submit only 1 of the following specimens:

 

Supplies:

M4-RT (T605)

Culturette (BBL Culture Swab) (T092)

Specimen Type: Swab

Sources: Cervix, urethra, urogenital, vaginal

Container/Tube:

Preferred: Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium [T092])

Acceptable: Swab in transport media: M4, M4-RT (T605), M5, M6, universal transport medium, or ESwab

Specimen Volume: One swab

Collection Instructions:

1. Vaginal: Collect specimen by swabbing back and forth over mucosa surface to maximize recovery of cells.

2. Urethra or Cervical: Collect specimen by inserting swab 1 to 3 cm and rotating 360 degrees.

3. Place swab back into swab cylinder.

 

Supplies: M4-RT (T605)

Specimen Type: Fluid

Sources: Amniotic, pelvic, prostatic secretion, reproductive drainage, semen

Container/Tube:

Preferred: Sterile container

Acceptable: Container with 3 mL of transport media: M4, M4-RT (T605), M5, M6 or universal transport media

Specimen Volume: 1-2 mL

 

Specimen Type: Urine, kidney/bladder stone, ureter

Container/Tube: Sterile container

Specimen Volume: 10 mL or entire specimen

 

Specimen Type: Tissue

Sources: Placenta, products of conception, genitourinary

Container/Tube: Sterile container

Specimen Volume: 5 mm

Collection Instructions: Submit only fresh tissue.


Specimen Minimum Volume

Pelvic Fluid, Amniotic Fluid, Prostatic Secretions, Semen, Reproductive Drainage or Fluid: 1 mL
Urine: 2 mL
Swab: 1 swab

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MGRP Mycoplasma genitalium PCR 69935-5

 

Result ID Test Result Name Result LOINC Value
SRC88 Specimen source 31208-2
32532 Mycoplasmoides genitalium PCR 69935-5

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.