Test ID MSCSF Bacteria, Virus, Fungus, and Parasite Metagenomic Sequencing, Spinal Fluid
Necessary Information
The following ask-at-order entry question must be answered at the time of test ordering:
Which CSF Collection Vial Number Sent (Required, Vial 1 is not acceptable)
Note: Answers may include "Vial 2", " Vial 3", or " Vial 4".
Test requests for specimens from "Vial 1" will be canceled. Test requests for submitted specimens with no response or response that does not specify the collection vial number will result in testing delays while the submitting facility is contacted to provide the information.
Specimen Required
Container/Tube: Sterile vial
Specimen Volume: 2 mL
Collection Instructions:
1. Masks should be worn by those collecting and processing specimens for this assay.
2. Handle all vials under sterile technique when open to the air.
3. A separate collection vial of CSF is preferred.
4. Submit specimen from collection vial 2, 3, or 4, as specimens from vial 1 are not acceptable.
5. Indicate on the label which vial is being submitted.
6. Do not centrifuge or heat inactivate.
Useful For
Detecting and identifying pathogenic organisms including bacteria, fungi, DNA viruses, RNA viruses, and parasites in cerebrospinal fluid
This test is not recommended as a test of cure because nucleic acids may persist after successful treatment.
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
SPID2 | Specimen Identification by PCR | No, (Bill Only) | No |
Testing Algorithm
For more information see the Meningitis/Encephalitis Panel Algorithm.
Method Name
Metagenomic Next-Generation Sequencing (NGS)
Reporting Name
Metagenomic Sequencing, CSFSpecimen Type
CSFSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
CSF | Frozen (preferred) | 21 days | |
Refrigerated | 7 days |
Reference Values
Negative.
No pathogenic DNA virus detected.
No pathogenic RNA virus detected.
No pathogenic parasite detected.
No pathogenic bacterium detected.
No pathogenic fungus detected.
Day(s) Performed
Monday through Friday
Report Available
7 to 14 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
MSCSF | Metagenomic Sequencing, CSF | 103566-6 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
MSCSF | Metagenomic Sequencing, CSF | 103566-6 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Special Instructions
CPT Code Information
0480U