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Test ID MSTC1 Strict Criteria Sperm Morphology for Infertility Diagnosis and Treatment, Semen

Useful For

Diagnosing male infertility


Selecting the most cost-effective therapy for treating male-factor infertility


Quantifying the number of germinal and WBCs per mL of semen

Method Name

Kruger Criteria Strict Morphology

Reporting Name

Strict Sperm Morphology, Slide

Specimen Type


Ordering Guidance

Conventional semen analysis (FER / Semen Analysis) should be performed in conjunction with each strict criteria sperm morphology.

Shipping Instructions

Send specimen Monday through Thursday only and not the day before a holiday.

Necessary Information

Sperm count/mL is required (million/mL).

Specimen Required

Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.

Container/Tube: Slides

Specimen Volume: 2 slides-10 microL of liquefied semen on each slide

Collection Instructions:

1. If sperm concentration is <10 million/mL, centrifuge the specimen at 300 x G for 10 minutes before making slides.

2 Label 2 frosted slides in pencil with the patient's first and last name and the date of specimen collection. No adhesive labels.

3. Allow the semen to liquefy for 30 minutes.

4. Place 10 microL of liquefied semen on the label end of each slide, and evenly smear the specimen using a plain slide (this process is the same as making a blood smear).

5. Allow the smears to air dry for 15 minutes before placing both slides into 1 slide holder for shipment.

Specimen Minimum Volume

A minimum count is needed; lab will determine

Specimen Stability Information

Specimen Type Temperature Time Special Container
Semen Ambient

Reference Values

Normal forms: ≥4.0%

Germinal cells/mL

<4 x 10(6) (normal)

≥4 x 10(6) (elevated germinal cells in semen are of unknown clinical significance)


<1 x 10(6) (normal)

≥1 x 10(6) (elevated white blood cells in semen are of questionable clinical significance)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
MSTC1 Strict Sperm Morphology, Slide 48812-2


Result ID Test Result Name Result LOINC Value
OVAL2 Strict Morph NL 10622-9
ACRSM Acrosom Defect 66494-6
HDSAB Head Shape Abnormal 66495-3
HDZAB Head Size Abnormal 66496-1
MD Midpiece Defect 10603-9
TAILD Tail Defect 10604-7
DBLF Double Forms 66497-9
MULTI Multiple Defects 66498-7
GERM3 Germ Cells/mL 10576-7
WBC6 WBC/mL 10579-1
CMT56 Comment 48767-8

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Report Available

2 to 4 days

NY State Approved