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Test ID MTBPZ Mycobacterium tuberculosis Complex, Pyrazinamide Resistance by pncA DNA Sequencing, Varies

Biohazard Infectious

Useful For

Detection of genotypic resistance to pyrazinamide by Mycobacterium tuberculosis complex isolates

Method Name

DNA Sequencing

Reporting Name

Mtb PZA Resistance, pncA Sequencing

Specimen Type


Shipping Instructions

1. See Infectious Specimen Shipping Guidelines in Special Instructions.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

Necessary Information

Specimen source and suspected organism identification are required.

Specimen Required

Specimen Type: Organism

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook 7H10 agar slant

Specimen Volume: Isolate

Collection Instructions: Organism must be in pure culture, actively growing.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)

Reference Values

Pyrazinamide resistance not detected

Day(s) and Time(s) Performed

1 day per week, Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87153-Mtb PZA Confirmation, pncA Sequence

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTBPZ Mtb PZA Resistance, pncA Sequencing 46245-7


Result ID Test Result Name Result LOINC Value
MTBPZ Mtb PZA Resistance, pncA Sequencing 46245-7

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

NY State Approved


Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MTBVP Mtb PZA Confirmation, pnc A Sequence No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex test may be performed and charged.


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.