Test ID MZIKV Zika Virus IgM Antibody Capture ELISA, Serum
Useful For
Screening for the presence of IgM-class antibodies to Zika virus
Highlights
This assay is a screening test for IgM-class antibodies to Zika virus. A presumptive positive result by this assay is not diagnostic for Zika virus infection and confirmatory testing may be required as determined by your local health department. Confirmatory testing by a molecular assay for detection of Zika virus RNA may also be considered.
This assay should be used in patients presenting at least 2 weeks post-symptom onset or last possible exposure to Zika virus. Reverse transcription-polymerase chain reaction (RT-PCR) for Zika virus RNA detection should be ordered in patients with less than 2 weeks of symptoms or postexposure.
A single negative result by the Zika IgM enzyme-linked immunosorbent assay (ELISA) should not be used to rule-out infection as the specimen may have been collected prior to the development of detectable antibodies.
Testing Algorithm
The following algorithms are available in Special Instructions:
Special Instructions
Method Name
IgM Antibody Capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA)
Reporting Name
Zika Virus MAC-ELISA, IgM, SSpecimen Type
SerumAdvisory Information
If the patient is pregnant, order PNZIK / Prenatal Zika Virus IgM Antibody Capture-ELISA, Serum.
For specimens collected less than 14 days post-symptom onset or possible Zika virus exposure, reverse transcription-polymerase chain reaction (RT-PCR) for Zika virus (ZIKV) on serum and urine to exclude a false-negative Zika virus IgM result is recommended. These are available as RZIKU / Zika Virus, PCR, Molecular Detection, Random, Urine and RZIKS / Zika Virus, PCR, Molecular Detection, Serum.
Additional Testing Requirements
This is a screening test for Zika virus. As required by your local health department, confirmatory testing of a presumptive positive result may be necessary.
Due to similar clinical presentation and cross reactivity, testing for IgM-class antibodies to dengue virus, concurrently with Zika virus IgM testing, is recommended, order DENVP / Dengue Virus Antibody/Antigen Panel, Serum.
Necessary Information
Three answers are required for order entry questions. The only acceptable answers are Yes or No.
If ordering electronically, record at order entry.
If not ordering electronically, provide the answers to the following questions with the specimen:
1. Could the patient be pregnant?
2. Has the patient traveled to or is a resident of a Zika endemic region?
3. Does the patient currently have or recently had Zika symptoms?
Specimen Required
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Specimen Minimum Volume
2 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Frozen | 30 days |
Reference Values
Negative
Day(s) and Time(s) Performed
Tuesday; 9 a.m.
Performing Laboratory

Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86794
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
MZIKV | Zika Virus MAC-ELISA, IgM, S | 80824-6 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
SZIKV | Zika Virus MAC-ELISA IgM, S | 80824-6 |
PREGO | Pregnant? | 11449-6 |
TRVL | Travel to/Resident of Zika Region? | 8691-8 |
SYMPS | Current or prior Zika symptoms? | 75325-1 |
NY State Approved
YesForms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.