Sign in →

Test ID OPATX Opiates Confirmation, Chain of Custody, Random, Urine

Useful For

Detection and quantification of codeine, hydrocodone, oxycodone, morphine, hydromorphone, oxymorphone, noroxycodone, noroxymorphone, norhydrocodone, dihydrocodeine, and naloxone in urine


Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited.

Method Name

Immunoassay followed by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Opiate Confirmation, CoC, U

Specimen Type


Specimen Required

Supplies: Chain-of-Custody Kit (T282)

Container/Tube: Chain-of-custody kit containing the specimen containers, seals, and documentation is required.

Specimen Volume: 5 mL

Collection Instructions: Collect urine specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.

Additional Information: Submitting less than 5 mL will compromise our ability to perform all necessary testing.

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Reference Values


Cutoff concentrations:



300 ng/mL


Liquid chromatography-tandem mass spectroscopy:

Codeine: 25 ng/mL

Dihydrocodeine: 25 ng/mL

Hydrocodone: 25 ng/mL

Norhydrocodone: 25 ng/mL

Hydromorphone: 25 ng/mL

Oxycodone: 25 ng/mL

Noroxycodone: 25 ng/mL

Oxymorphone: 25 ng/mL

Noroxymorphone: 25 ng/mL

Naloxone: 25 ng/mL

Morphine: 25 ng/mL

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information



G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OPATX Opiate Confirmation, CoC, U In Process


Result ID Test Result Name Result LOINC Value
6541 Opiates Immunoassay Screen 70151-6
36213 Codeine-by LC-MS/MS 16250-3
42005 Dihydrocodeine-by LC-MS/MS 19448-0
36214 Hydrocodone-by LC-MS/MS 16252-9
42006 Norhydrocodone-by LC-MS/MS 61422-2
36215 Hydromorphone-by LC-MS/MS 16998-7
36216 Oxycodone-by LC-MS/MS 16249-5
42007 Noroxycodone-by LC-MS/MS 61425-5
36212 Oxymorphone-by LC-MS/MS 17395-5
42008 Noroxymorphone-by LC-MS/MS 90894-7
42009 Naloxone-by LC-MS/MS 77207-9
36217 Morphine-by LC-MS/MS 16251-1
36218 Opiates Interpretation 18390-5
36219 Chain of Custody 77202-0

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Additional Tests

Test ID Reporting Name Available Separately Always Performed
COCH Chain of Custody Processing No Yes
ADLTX Adulterants Survey, CoC, U Yes Yes

Testing Algorithm

Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.

Report Available

3 to 5 days

NY State Approved



1. Chain of Custody Request is included in the Chain-of-Custody Kit (T282).

2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.