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Test ID PBORR Lyme Disease, Molecular Detection, PCR, Varies

Useful For

Supporting the diagnosis of Lyme disease in conjunction with serologic testing

 

Specific indications including testing skin biopsies when a rash lesion is not characteristic of erythema migrans, and testing synovial fluid or synovium to support the diagnosis of Lyme arthritis

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Lyme Disease PCR

Specimen Type

Varies


Ordering Guidance


This assay does not detect Borrelia miyamotoi. If infection with this organism is suspected, order BMIYB / Borrelia miyamotoi Detection PCR, Blood or BMIYC / Borrelia miyamotoi Detection PCR, Spinal Fluid.



Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Label specimen as spinal fluid.

 

Specimen Type: Synovial fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

Collection Instructions: Label specimen as synovial fluid.

 

Specimen Type: Tissue (fresh only)

Sources: Skin or synovial biopsy

Container/Tube: Sterile container with normal saline

Specimen Volume: Approximately 4 mm(3)

Collection Instructions:

1. Submit only fresh tissue.

2. Skin biopsies:

a. Wash biopsy site with an antiseptic soap. Thoroughly rinse area with sterile water. Do not use alcohol or iodine preparations. A local anesthetic may be used.

b. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.

3. Label specimen with source of tissue.


Specimen Minimum Volume

Spinal Fluid, Synovial Fluid: 0.3 mL
Tissue: NA

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Day(s) Performed

June through November: Monday through Saturday

December through May: Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87476

87798 x 2

87999 (if appropriate for government payers)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PBORR Lyme Disease PCR 94253-2

 

Result ID Test Result Name Result LOINC Value
SRC71 Specimen Source 31208-2
23635 B. burgdorferi PCR 94250-8
38288 B. mayonii PCR 94251-6
38289 B. garinii/B. afzelii PCR 94252-4
38323 Lyme CSF Comment 59464-8

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Testing Algorithm

The following algorithms are available in Special Instructions:

-Acute Tick-Borne Disease Testing Algorithm

-Meningitis/Encephalitis Panel Algorithm

Report Available

1 to 4 days

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.