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Test ID PCPDS Plasma Cell Proliferative Disorder, FISH, Bone Marrow


Ordering Guidance


This assay detects high risk abnormalities plus CCND1/IGH fusion observed in the bone marrow of patients with a plasma cell disorder.

-For a more complete genetic evaluation, order MPCDS / mSMART, Plasma Cell Proliferative Disorder, FISH, Bone Marrow.

-For paraffin-embedded tissue specimens, order PLASF / Plasma Cell Proliferative Disorder, FISH, Tissue.

-For fixed cell pellet specimens, order MFCF / Myeloma, FISH, Fixed Cells.

-Testing will be changed to the appropriate test if this test is ordered on either of the previous specimens or if bone marrow specimens are received more than 96 hours from collection.



Shipping Instructions


1. Specimen should arrive within 96 hours of collection.

2. Advise Express Mail or equivalent if not on courier service.



Necessary Information


1. Provide a reason for testing with each specimen. The laboratory will not reject testing if this information is not provided but appropriate testing and interpretation may be compromised or delayed.

2. A pathology and/or flow cytometry report may be requested to optimize testing and aid in interpretation of results.



Specimen Required


Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: Lavender top (EDTA) or green top (heparin)

Specimen Volume: 4 mL

Collection Instructions: Invert several times to mix bone marrow


Useful For

Aiding in the diagnosis of new cases of multiple myeloma or other plasma cell proliferative disorders

 

Identifying prognostic markers based on the abnormalities found

Additional Tests

Test ID Reporting Name Available Separately Always Performed
CSPCF PCPDS Pre-Analysis Cell Sorting, BM No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_PBCT Probe, +2 No, (Bill Only) No
_PADD Probe, +1 No, (Bill Only) No
_PB02 Probe, +2 No, (Bill Only) No
_PB03 Probe, +3 No, (Bill Only) No
_IL25 Interphases, <25 No, (Bill Only) No
_I099 Interphases, 25-99 No, (Bill Only) No
_I300 Interphases, >=100 No, (Bill Only) No

Testing Algorithm

This test is designed for diagnostic specimens from patients with multiple myeloma or other plasma cell proliferative disorders. If a request for testing has been submitted within 12 months of a complete and informative plasma cell proliferative disorder fluorescence in situ hybridization (FISH) study, the current test request will be cancelled.

 

This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.

 

When this test is ordered, pre-analysis cell sorting will be performed at an additional charge.

 

For diagnostic samples, all probes in the initial panel will be evaluated if sufficient plasma cells are identified. The initial panel includes testing for the following abnormalities using the probes listed:

17p-, TP53/D17Z1

1q gain, TP73/1q22

14q32 rearrangement, IGH

 

Based on the results from the initial panel, reflex testing may be performed to identify the following abnormalities using the probes listed:

t(11;14), CCND1/IGH

t(14;16)(q32;q23) IGH/MAF

t(4;14)(p16.3;q32) FGFR3/IGH

t(14;20)(q32;q12) IGH/MAFB 

 

For follow-up samples, only TP73/1q22, TP53/D17Z1 and MYC probes will be tested. If a diagnostic sample was uninformative for a probe set due to an insufficient number of plasma cells, attempts may be made to achieve results for the missing probe on a subsequent sample (if sufficient plasma cells are identified).

 

Initial screening will be performed to determine if sufficient plasma cells are present within the provided specimen.

 

If the specimen is received greater than 96 hours from collection, or arrives clotted or hemolyzed, this test will be canceled and MFCF / Myeloma, FISH, Fixed Cells will be added as the more appropriate test.

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

Plasma Cell Prolif, Sort, FISH

Specimen Type

Bone Marrow

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Bone Marrow Ambient (preferred) 4 days
  Refrigerated  4 days

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Report Available

7 to 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88271x2, 88291-DNA probe, each (first probe set), Interpretation and report

88271x2-DNA probe, each; each additional probe set (if appropriate)

88271x1-DNA probe, each; coverage for sets containing 3 probes (if appropriate)

88271x2-DNA probe, each; coverage for sets containing 4 probes (if appropriate)

88271x3-DNA probe, each; coverage for sets containing 5 probes (if appropriate)

88274 w/modifier 52-Interphase in situ hybridization, <25 cells, each probe set (if appropriate)

88274-Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)

88275-Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PCPDS Plasma Cell Prolif, Sort, FISH 98014-4

 

Result ID Test Result Name Result LOINC Value
606080 Result Summary 62357-9
606081 Interpretation 69965-2
606082 Result Table 93356-4
606083 Result 62356-1
GC054 Reason for Referral 42349-1
606084 Specimen 31208-2
606085 Source 39111-0
606086 Method 85069-3
606087 Additional Information 48767-8
606088 Disclaimer 62364-5
606089 Released By 18771-6

NY State Approved

Yes