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Test ID PEE Porphyrins Evaluation, Whole Blood

Useful For

Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria

Method Name


Reporting Name

Porphyrins Evaluation, WB

Specimen Type

Whole blood

Ordering Guidance

This is the preferred test for assessment for protoporphyria. The preferred test for assessing lead toxicity in children is blood lead. For more information see PBDV / Lead, Venous, with Demographics, Blood or PBDC / Lead, Capillary, with Demographics, Blood. The preferred screening test for suspicion of a hepatic porphyria is urine porphyrins. For more information see PQNRU / Porphyrins, Quantitative, Random, Urine.

Necessary Information

Include a list of medications the patient is currently taking.

Specimen Required

All porphyrin tests on whole blood can be performed on 1 collection tube.


Patient Preparation: Patient should abstain from alcohol for 24 hours.


Preferred: Green top (sodium heparin)

Acceptable: Dark blue top (metal free heparin), green top (lithium heparin), lavender top (EDTA)

Specimen Volume: Full tube

Collection Instructions: Immediately place specimen on wet ice.

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Refrigerated 7 days

Reference Values


<80 mcg/dL

Day(s) Performed

Monday, Wednesday, Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information


82542-if appropriate

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PEE Porphyrins Evaluation, WB 2814-2


Result ID Test Result Name Result LOINC Value
88886 Total Porphyrins, WB 2814-2
29356 Interpretation 59462-2

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
PPFE Protoporphyrins, Fractionation, WB Yes No

Testing Algorithm

This test is recommended for screening patients for possible erythropoietic protoporphyria and X-linked dominant protoporphyria. In addition, it can be used for evaluation of iron-deficiency anemia and chronic lead intoxication. Testing begins with total erythrocyte porphyrins. If the result is below 80 mcg/dL, it is normal and testing is complete.


If the total erythrocyte porphyrin value is 80 mcg/dL or above, the protoporphyrin fractionation assay will automatically be performed at an additional charge. The fractionation test results include noncomplexed (free) protoporphyrin and zinc-complexed protoporphyrin.


The following algorithms are available in Special Instructions:

-Porphyria (Acute) Testing Algorithm 

-Porphyria (Cutaneous) Testing Algorithm 


Report Available

3 to 4 days

NY State Approved