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Test ID PFGE Bacterial Typing by Pulsed-Field Gel Electrophoresis, Varies

Biohazard Infectious

Useful For

Investigating infection outbreaks by a single bacterial species

Method Name

Pulsed-Field Gel Electrophoresis

Reporting Name

Bacterial Typing

Specimen Type

Varies


Advisory Information


This test compares bacterial strains of non-aureus Staphylococcus species; non-pneumophila Legionella species; Enterobacteriaceae other than Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, and Serratia marcescens; nonfermenting Gram-negative bacilli other than Acinetobacter baumannii and Pseudomonas aeruginosa; Listeria species, Bacillus species, Enterococcus species other than E faecalis and E faecium, Streptococcus species other than S pyogenes, Lactobacillus species, and Clostridium perfringens.

 

For Acinetobacter baumannii, Campylobacter jejuni/coli, Clostridioides (Clostridium) difficile, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Legionella pneumophila, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, or Streptococcus pyogenes isolates, order BTWGS / Bacterial Typing by Whole Genome Sequencing, Varies.



Shipping Instructions


1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place isolates in a large infectious container (T146) and label as an etiologic agent/infectious substance.

3. Place all pulsed-field gel electrophoresis (PFGE) isolates (all patients and/or sites to be compared) together in 1 large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.



Necessary Information


Organism identification and specimen source are required.

Results will be faxed to the client.



Specimen Required


Supplies: Infectious Container, Large (T146)

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants in pure culture

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.

Additional Information:

1. Each slant must be submitted under a separate order.

2. Original isolates should be saved at the client site if additional pulsed-field gel electrophoresis testing may be needed (eg, to compare to future outbreak isolates that may occur).


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Reported as isolates are "indistinguishable" or "different" by pulsed-field gel electrophoresis.

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87152

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PFGE Bacterial Typing 42661-9

 

Result ID Test Result Name Result LOINC Value
PFGE Bacterial Typing 42661-9

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.