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Test ID PFGE Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

Biohazard Infectious

Useful For

Investigating infection outbreaks by a single bacterial species

Method Name

Pulsed-Field Gel Electrophoresis of DNA Fragments

Reporting Name

Bacterial Typing

Specimen Type

Varies


Advisory Information


This test compares bacterial strains of non-aureus Staphylococcus species; non-pneumophila Legionella species; Enterobacteriaceae other than Klebsiella pneumoniae; non-fermenting Gram-negative bacilli other than Acinetobacter baumannii; Listeria species, Bacillus species, Enterococcus species, Streptococcus species, Lactobacillus species, and Clostridium perfringens.

 

For Staphylococcus aureus, Acinetobacter baumannii, Klebsiella pneumoniae, Legionella pneumophila or Clostridioides (Clostridium) difficile isolates, order BTWGS / Bacterial Typing by Whole Genome Sequencing.



Shipping Instructions


1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place isolates in a large infectious container (T146) and label as an etiologic agent/infectious substance.

3. Place all pulsed-field gel electrophoresis (PFGE) isolates (all patients and/or sites to be compared) together in 1 large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.



Necessary Information


Organism identification and specimen source are required for processing.

Results will be faxed to the client.



Specimen Required


Supplies: Infectious Container, Large (T146)

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants in pure culture

Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.

Additional Information:

1. Each slant must be submitted under a separate order.

2. Original isolates should be saved at the client site if additional PFGE testing may be needed (eg, to compare to future outbreak isolates that may occur).


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Reported as isolates are "indistinguishable" or "different" by pulsed-field gel electrophoresis.

Day(s) and Time(s) Performed

Monday through Friday; Continuously

Performing Laboratory

Mayo Medical Laboratories in Rochester

CPT Code Information

87152

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PFGE Bacterial Typing In Process

 

Result ID Test Result Name Result LOINC Value
PFGE Bacterial Typing In Process

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.