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Test ID PMND1 Primary Membranous Nephropathy Diagnostic Cascade, Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Distinguishing primary from secondary membranous nephropathy using an algorithmic approach

 

Monitoring patients with membranous nephropathy at very low antibody titers

 

Screening for anti-phospholipase A2 receptor antibodies

Profile Information

Test ID Reporting Name Available Separately Always Performed
EURO Phospholipase A2 Receptor, ELISA, S Yes, (Order PLA2M) Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
PLA2I PLA2R, Immunofluorescence, S Yes No
THSD7 THSD7A Ab, S Yes No

Testing Algorithm

The phospholipase A2 receptor (PLA2R) enzyme-linked immunosorbent assay (ELISA) is initially performed.

 

If the PLA2R ELISA result is less than 20, then the PLA2R immunofluorescence testing will be performed at an additional charge.

 

If the PLA2R immunofluorescence result is negative, thrombospondin type-1 domain-containing 7A (THSD7A) antibody testing will be performed at an additional charge.

Method Name

EURO: Enzyme-Linked Immunosorbent Assay (ELISA)

PLA2I, THSD7: Indirect Immunofluorescence Assay (IFA)

Reporting Name

Prim Membranous Nephropathy Diag, S

Specimen Type

Serum

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  8 hours

Reference Values

ANTI-PHOSPHOLIPASE A2 RECEPTOR (PLA2R) ENZYME-LINKED IMMUNOSORBENT ASSAY:

<14 RU/mL: Negative

14 to 19 RU/mL: Borderline

≥20 RU/mL: Positive

 

PLA2R IMMUNOFLUORESCENCE:

Negative

 

THROMBOSPONDIN TYPE-1 DOMAIN-CONTAINING 7A ANTIBODIES:

Negative

Day(s) Performed

Monday, Wednesday, Friday

Report Available

3 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

See Individual Test IDs

CPT Code Information

83520

86255 (x1 or x2, if applicable)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PMND1 Prim Membranous Nephropathy Diag, S 73737-9

 

Result ID Test Result Name Result LOINC Value
EURO Phospholipase A2 Receptor, ELISA, S 73737-9

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.