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Test ID PTOX Toxoplasma gondii, Molecular Detection, PCR, Varies

Useful For

Supporting the diagnosis of acute cerebral, ocular, disseminated, or congenital toxoplasmosis


This test should not be used to screen healthy patients.

Method Name

Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Toxoplasma gondii PCR

Specimen Type


Specimen Required

Necessary Information

Specimen source is required.

Specimen Required

Submit only 1 of the following specimens:

Specimen Type: Amniotic fluid

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.


Specimen Type: Spinal fluid

Supplies: Sarstedt 5 mL Aliquot Tube (T914)


Preferred: 12 x 75-mm screw cap vial

Acceptable: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.


Specimen Type: Fresh tissue


-M4-RT (T605)

-Bartels FlexTrans VTM-3 mL (T892)

-Jiangsu VTM-3 mL (T891)


Preferred: Multi-microbe medium (eg, M4-RT)

Acceptable: Sterile container with 1 to 2 mL of sterile saline

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue in a sterile container containing 1 mL to 2 mL of sterile saline or multi-microbe medium (M4-RT, M4, or M5)


Specimen Type: Ocular fluid

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container: 12 x 75-mm screw cap vial

Specimen Volume: 0.3 mL

Collection Instructions:

1. Aliquot collected fluid into screw-cap vial. Do not submit ocular fluid in syringe.

2. Do not centrifuge or dilute the specimen.

Specimen Minimum Volume

Amniotic Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL
Tissue: 2 × 2 mm biopsy

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values


Day(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
PTOX Toxoplasma gondii PCR 42626-2


Result ID Test Result Name Result LOINC Value
SRC74 Specimen Source 31208-2
81795 Toxoplasma gondii PCR 42626-2

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Report Available

Same day/1 to 4 days

NY State Approved



If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Testing Algorithm

See Meningitis/Encephalitis Panel Algorithm in Special Instructions.