Sign in →

Test ID QNKS Natural Killer (NK)/Natural Killer T-Cell Subsets, Quantitative, Blood

Useful For

Quantitation of the major natural killer (NK)-cell subsets relative to total NK cells (NK cell subsets) or total lymphocytes (NK T cells)


Assessment in the following clinical contexts: HIV, primary immune deficiencies with NK cell defects, NK-cell lymphocytosis, solid-organ transplantation, immune reconstitution following bone marrow or hematopoietic cell transplantation


This test is not useful for diagnosis or classification of NK cell malignancies.


This test should not be used for assessing NK cell cytotoxic function.

Method Name

Flow Cytometry

Reporting Name

Quantitative NK/NKT Subsets

Specimen Type

WB Sodium Heparin

Ordering Guidance

This assay does not measure cell-surface or intracellular proteins on natural killer or natural killer T-cell subsets.


A minimum CD45 lymph count (as measured by flow cytometry in the laboratory) is required to report this test. If that requirement is not met (eg, patients with severe lymphopenia), the test will be canceled and an alternate test will be suggested (TBBS / Quantitative Lymphocyte Subsets: T, B, and Natural Killer [NK] Cells, Blood).

Shipping Instructions

Send specimen Monday through Thursday only. Specimen must arrive within 24 hours of collection and by 10 a.m. on Friday. Collect and package specimen as close to shipping time as possible. Ship specimen overnight.

Necessary Information

Ordering physician's name and phone number are required.

Specimen Required

Container/Tube: Green top (sodium heparin)

Specimen Volume: 3 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

Additional Information: For serial monitoring, it is recommended that specimens are collected at the same time of day.

Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
WB Sodium Heparin Ambient 30 hours GREEN TOP/HEP

Reference Values

The appropriate age-related reference values will be provided on the report. Pediatric reference values are not available for patients less than 7 years of age and therefore, interpretation will be based on the 7 to 17-year-old ranges with appropriate cautionary statements in the interpretation.

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86356 x3



LOINC Code Information

Test ID Test Order Name Order LOINC Value
QNKS Quantitative NK/NKT Subsets 98073-0


Result ID Test Result Name Result LOINC Value
QNKSI Quantitative NK/NKT Interpretation 69052-9
NKA45 Total Lymphs (CD45+) 27071-0
NKP3N % Total CD45+CD3- cells 89311-5
NKA3N Total CD45+CD3- cells 89313-1
NKP % Total NK Cells 8112-5
NKA Total NK Cells 9728-7
NKPCY % Cytotoxic NK (CD16++CD56+) 42189-1
NKACY Cytotoxic NK (CD16++CD56+) 42188-3
NK56P % Cytokine-Producing NK (CD56++) 8133-1
NK56A Cytokine-Producing NK (CD56++) 14113-5
NKPL3 % Total CD45+CD3+ Cells 56907-9
NKAL3 Total CD45+CD3+ Cells 89312-3
NKTP % NKT cells (CD3+CD56+) 17135-5
NKTA NKT cells (CD3+CD56+) 26858-1

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Report Available

3 to 4 days

NY State Approved


Disease States

  • HIV infection