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Test ID SLYME Lyme Antibody Modified 2-Tier with Reflex, Serum

Specimen Required

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: 5-mL aliquot tube

Specimen Volume: 0.6 mL

Useful For

Diagnosis of Lyme disease


This test should not be used as a screening procedure for the general population.

Testing Algorithm

If the Lyme antibody result is positive or equivocal, then confirmation by whole cell sonicate will be performed at an additional charge.


See Acute Tick-Borne Disease Testing Algorithm in Special Instructions.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Lyme Ab Modified 2-Tier w/Reflex, S

Specimen Type


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 10 days
  Frozen  30 days

Reference Values


Reference values apply to all ages.

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information


86617 x 2-Lyme IgM/IgG, WCS, EIA S (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SLYME Lyme Ab Modified 2-Tier w/Reflex, S 83081-0


Result ID Test Result Name Result LOINC Value
SLYME Lyme Ab Modified 2-Tier w/Reflex, S 83081-0

NY State Approved


Reflex Tests

Test ID Reporting Name Available Separately Always Performed
TLYME Lyme IgM/IgG, WCS, EIA, S Yes No


If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.