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HelpTest ID STICK Tick-Borne Antibodies, Modified 2-Tier, ELISA, Serum
Ordering Guidance
During the acute phase of an Anaplasma phagocytophilum or Ehrlichia chaffeensis infection, serologic tests are often nonreactive, polymerase chain reaction (PCR) testing is available to aid in the diagnosis of these cases; see EHRL / Ehrlichia/Anaplasma, Molecular Detection, PCR, Blood.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1.35 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluation of the most common tick-borne diseases found in the United States, including Lyme disease, human monocytic and granulocytic ehrlichiosis, and babesiosis using the modified 2-tier testing algorithm approach
Evaluation of patients with a history of, or suspected, tick exposure who are presenting with fever, myalgia, headache, nausea, and other nonspecific symptoms
Sero-epidemiological surveys of the prevalence of the infection in certain populations
Diagnosis of Lyme disease
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ANAP | Anaplasma phagocytophilum Ab, IgG,S | Yes | Yes |
| EHRC | Ehrlichia Chaffeensis (HME) Ab, IgG | Yes | Yes |
| BABG | Babesia microti IgG Ab, S | Yes | Yes |
| SLYME | Lyme Ab Modified 2-Tier w/Reflex, S | Yes | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TLYME | Lyme IgM/IgG, WCS, EIA, S | Yes | No |
Testing Algorithm
If the Lyme antibody result is positive or equivocal, then Lyme disease antibody confirmation will be performed at an additional charge.
Special Instructions
Method Name
ANAP, EHRC, BABG: Immunofluorescence Assay (IFA)
SLYME: Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
Tick-Borne Abs w/ Lyme MTTTA, SSpecimen Type
SerumSpecimen Minimum Volume
1.1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 10 days | |
| Frozen | 14 days |
Reference Values
Anaplasma phagocytophilum ANTIBODY, IgG
<1:64
Reference values apply to all ages.
Ehrlichia chaffeensis (HME) ANTIBODY, IgG
<1:64
Reference values apply to all ages.
Babesia microti IgG ANTIBODIES
<1:64
Reference values apply to all ages.
LYME ANTIBODY
Negative
Reference values apply to all ages.
Day(s) Performed
Monday through Friday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
See Individual Test IDsCPT Code Information
86618
86666 x 2
86753
86617 x 2 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| STICK | Tick-Borne Abs w/ Lyme MTTTA, S | 103603-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 81157 | Anaplasma phagocytophilum Ab, IgG,S | 23877-4 |
| 81128 | Babesia microti IgG Ab, S | 16117-4 |
| 81478 | Ehrlichia Chaffeensis (HME) Ab, IgG | 47405-6 |
| SLYME | Lyme Ab Modified 2-Tier w/Reflex, S | 83081-0 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.