Sign in →

Test ID TDP Thiamine (Vitamin B1), Whole Blood

Useful For

Assessment of thiamine deficiency

 

Measuring thiamine levels in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Thiamin (Vitamin B1), WB

Specimen Type

Whole Blood EDTA


Shipping Instructions


Ship specimen in amber vial to protect from light.



Specimen Required


Patient Preparation: Fasting overnight (12-14 hours). Infants-draw prior to next feeding. Water can be taken as needed.

Supplies: Amber Frosted Tube, 5 mL (T192)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Amber vial

Specimen Volume: 4 mL

Collection Instructions:

1. Invert 8 to 10 times to mix blood.

2. Transfer whole blood into amber vial or tube and freeze within 24 hours of collection.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Frozen 28 days LIGHT PROTECTED

Reference Values

70-180 nmol/L

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

84425

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TDP Thiamin (Vitamin B1), WB 32554-8

 

Result ID Test Result Name Result LOINC Value
85753 Thiamin (Vitamin B1), WB 32554-8

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Highlights

-Whole blood thiamine testing is superior to currently available alternative tests for assessing thiamine status. Serum or plasma thiamine testing suffers from poor sensitivity and specificity, and less than 10% of blood thiamine is contained in plasma.

-Thiamine diphosphate (TDP) is the active form of thiamine and is most appropriately measured to assess thiamine status. Thiamine diphosphate in circulating blood is present in erythrocytes, but is undetectable (present in very low levels) in plasma or serum.

-LC-MS/MS analysis of TDP in whole blood is the most sensitive, specific, and precise method for determining the nutritional status of thiamine and is a reliable indicator of total body stores.

-This assay specifically targets and quantitates the active form of thiamine, TDP, as an indicator of thiamine status.