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Test ID TLYM T-Cell Lymphoma, FISH, Tissue


Advisory Information


This assay detects chromosome abnormalities observed in paraffin-embedded tissue samples of patients with T-cell lymphoma. For testing the blood and bone marrow from patients with T-cell lymphoma, see TLPF / T-Cell Lymphoma, FISH, Blood or Bone Marrow.



Shipping Instructions


Advise Express Mail or equivalent if not on courier service.



Necessary Information


Provide a reason for referral and pathology report with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Lymph node

Preferred: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

 

Acceptable: Slides

Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.

 

Specimen Type: Solid tumor

Preferred: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.

 

Acceptable: Slides

Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.


Useful For

Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas

 

Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphomas

 

Providing potentially prognostic information in patients with documented systemic anaplastic lymphoma kinase-negative anaplastic large cell lymphoma

 

Supporting the diagnosis of peripheral T-cell lymphoma when coordinated with consultation by anatomic pathology

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
_PRAH Probe, Each Additional (TLYM) No, (Bill Only) No

Testing Algorithm

This test does not include a pathology consultation. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.

 

A charge and CPT code is applied for each probe set hybridized, analyzed, and reported.

 

Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the Clinical Information.

 

If the patient is being tracked for known abnormalities, indicate which probes should be used.

 

Panel includes testing for the following abnormalities using the probes listed:

14q32.1 rearrangement, TCL1A

14q11.2 rearrangement, TRAD

-7/7q-/i(7q), D7S486/D7Z1

+8, D8Z2/MYC

2p23.2 rearrangement, ALK

3q28 rearrangement, TP63 and TBL1XR1/TP63 (only performed when necessary to resolve or confirm TP63 rearrangement concerns)

6p25.3 rearrangement, IRF4 (DUSP22

Method Name

Fluorescence In Situ Hybridization (FISH)

Reporting Name

T-cell Lymphoma, FISH, Tissue

Specimen Type

Tissue

Specimen Minimum Volume

For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides. Include 1 hematoxylin and eosin-stained slide.

Specimen Stability Information

Specimen Type Temperature Time
Tissue Ambient (preferred)
  Refrigerated 

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Samples processed Monday through Sunday. Results reported Monday through Friday 8 a.m.-5 p.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88377-if 1 probe set

88377 x 2-if 2 probe sets

88377 x 3-if 3 probe sets

88377 x 4-if 4 probe sets

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TLYM T-cell Lymphoma, FISH, Tissue In Process

 

Result ID Test Result Name Result LOINC Value
603140 Result Summary 50397-9
603141 Interpretation 69965-2
603142 Result Table 36908-2
603143 Result 62356-1
GC040 Reason for Referral 42349-1
603144 Specimen 31208-2
603145 Source 31208-2
603146 Tissue ID 80398-1
603147 Method 85069-3
603148 Additional Information 48767-8
603149 Disclaimer 62364-5
603150 Released By 18771-6