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Test ID TLYME Lyme IgM and IgG, Whole Cell Sonicate, ELISA, Serum

Ordering Guidance

This test should only be ordered on specimens that have tested positive or equivocal by a first tier Lyme disease antibody test.

Specimen Required

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Useful For

Supplemental testing for samples with positive or equivocal first-tier test results for antibodies to Lyme disease causing Borrelia species


This test should not be used as a screening procedure for the general population.


Lyme disease serology positive results by the modified 2-tier testing algorithm are supportive evidence for the presence of antibodies and exposure to Borrelia burgdorferi, the cause of Lyme disease.

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Lyme IgM/IgG, WCS, EIA, S

Specimen Type


Specimen Minimum Volume

0.50 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 10 days
  Frozen  30 days

Reference Values


Reference values apply to all ages.

Day(s) Performed


Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86617 x2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TLYME Lyme IgM/IgG, WCS, EIA, S 34942-3


Result ID Test Result Name Result LOINC Value
LYMEM Lyme Ab, IgM, S 40612-4
LYMEG Lyme Ab, IgG, S 16480-6
LYMEI Lyme Ab Interpretation 46248-1

NY State Approved



If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.