Test ID TREGS T-Cell Subsets, Regulatory (Tregs), Blood
Useful For
Evaluating patients with clinical features of IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked inheritance) and other primary immunodeficiencies, autoimmune diseases, allergy and asthma, and graft-vs-host disease post-hematopoietic stem cell transplantation
Method Name
Flow Cytometry
Reporting Name
T Cell Subsets, Regulatory (Tregs)Specimen Type
Whole Blood EDTAShipping Instructions
Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Collect and package specimen as close to shipping time as possible.
It is recommended that specimens arrive within 24 hours of collection.
Samples arriving on the weekend and observed holidays may be canceled.
Necessary Information
Ordering physician's name and phone number are required.
Specimen Required
For serial monitoring, it is recommended that specimens are collected at the same time of day.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Reference Values
The appropriate age-related reference values will be provided on the report.
Day(s) Performed
Monday through Friday
Specimens are required to be received in the lab weekdays and by 4 p.m. on Friday. No weekend processing.
Performing Laboratory

CPT Code Information
86359
86361
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TREGS | T Cell Subsets, Regulatory (Tregs) | 90413-6 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
609282 | CD4 (T Cells) | 24467-3 |
29143 | % Activated CD4+ T cells (4+CD25+) | 13332-2 |
29144 | % Natural Tregs | 89320-6 |
29145 | % N. Naive Tregs | 89319-8 |
29146 | % CD4+CD25-CD127+ (Tr1/Th3) | 89318-0 |
29147 | Activated CD4+ T cells (4+CD25+) | 26982-9 |
29148 | CD4+CD25+CD127loCD45RO+ (Nat Tregs) | 89316-4 |
29149 | CD4+CD25+CD127loCD45RA+ Naive Tregs | 89315-6 |
29150 | CD4+CD25-CD127+ (Tr1/Th3) | 89317-2 |
29177 | Interpretation | 69052-9 |