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Test ID TREGS T-Cell Subsets, Regulatory (Tregs)

Useful For

Evaluating patients with clinical features of IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked inheritance) and other primary immunodeficiencies, autoimmune diseases, allergy and asthma, and graft-vs-host disease post-hematopoietic stem cell transplantation

Method Name

Flow Cytometry

Reporting Name

T Cell Subsets, Regulatory (Tregs)

Specimen Type

Whole Blood EDTA


Shipping Instructions


Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of draw.

 

Specimens arriving on the weekend may be canceled.



Necessary Information


Ordering physician's name and phone number are required.



Specimen Required


For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Ambient 72 hours

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) and Time(s) Performed

Monday through Friday

Do not send specimen after Thursday. Specimen must be received by 10 a.m. on Friday.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86359-T cells, total count

86361-Absolute CD4 count

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TREGS T Cell Subsets, Regulatory (Tregs) 90413-6

 

Result ID Test Result Name Result LOINC Value
29143 % Activated CD4+ T cells (4+CD25+) 13332-2
29144 % Natural Tregs 89320-6
29145 % N. Naive Tregs 89319-8
29146 % CD4+CD25-CD127+ (Tr1/Th3) 89318-0
29147 Activated CD4+ T cells (4+CD25+) 26982-9
29148 CD4+CD25+CD127loCD45RO+ (Nat Tregs) 89316-4
29149 CD4+CD25+CD127loCD45RA+ Naive Tregs 89315-6
29150 CD4+CD25-CD127+ (Tr1/Th3) 89317-2
29177 Interpretation 69052-9

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.