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Test ID TVRNA Trichomonas vaginalis, Nucleic Acid Amplification, Female Patients, Varies

Useful For

Detection of Trichomonas vaginalis in female patients

Method Name

Transcription Mediated Amplification

Reporting Name

Trichomonas vaginalis Amplified RNA

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


This test is performed only on female patients.

 

Submit only 1 of the following specimens:

 

Specimen Type: Endocervix

Supplies: Swab, Aptima Male/Female Collection (T583) (also known as APTIMA Collection Unisex Swab)

Specimen Volume: Adequate amount

Collection Instructions:

1. Endocervix specimens must be collected using the APTIMA Collection Unisex Swab (T583)

2. Use cleaning swab (white shaft) to remove excess mucus from endocervix and discard.

3. Insert second swab (blue shaft) 1 to 1.5 cm into endocervical canal, and rotate swab gently for 30 seconds. Avoid touching vaginal wall when removing swab.

4. Place second swab (blue shaft) into APTIMA transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

5. Cap tube securely, and label tube with patient's entire name, and date and time of collection.

Specimen Stability Information: Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature) within 60 days of collection. If longer storage is needed, freeze at -20 to -70° C for up to 180 days.

 

Specimen Type: Vaginal

Supplies: Swab, Aptima Multitest Swab Specimen Collection Kit (T584)

Specimen Volume: Adequate amount

Collection Instructions:

1. Vaginal specimens must be collected using the APTIMA Multitest Swab Specimen Collection Kit (T584), formerly called Aptima Vaginal Swab Specimen Collection Kit).

2. Insert swab (pink shaft) about 5 cm past introitus and rotate gently for 30 seconds.

3. Place swab into APTIMA transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.

4. Cap tube securely, and label tube with patient's entire name, and date and time of collection.

Specimen Stability Information: Transport and store swab container at 2 to 30° C (refrigerate is preferred temperature) within 60 days of collection. If longer storage is needed, freeze at -20 to -70° C for up to 180 days.

 

Specimen Type: Urine

Supplies: Aptima Urine Transport Tube (T582)

Container/Tube: APTIMA Urine Specimen Transport Tube (T582)

Specimen Volume: 15-20 mL

Collection Instructions:

1. Patient should not have urinated for at least 1 hour prior to specimen collection.

2. Patient should collect first portion of random voided urine (first part of stream) into a sterile, plastic, preservative-free container.

3. Transfer 2 mL of urine into the APTIMA urine specimen transport tube using the disposable pipette provided within 24 hours of collection. The correct volume of urine has been added when the fluid level is between the black fill lines on the APTIMA urine transport tube. Place the labels on the transport tube so the black fill lines are still visible for volume confirmation.

Specimen Stability Information: Transport and store urine specimen transport tube at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. If longer storage is needed, freeze at -20 to -70° C for up to 180 days.

 

Specimen Type: ThinPrep Specimen (Endocervix)

Supplies: Aptima Thin Prep Transport Tube (T652)

Container/Tube: ThinPrep (also called PreservCyt) Collection Kit

Specimen Volume: 1 mL

Collection Instructions:

1. Aliquot ThinPrep specimen for Trichomonas and/or Chlamydia and/or Neisseria testing before processing for Pap smear. For each specimen, use a new pair of clean gloves.

2. Vortex ThinPrep/PreservCyt vial 3 to 10 seconds. Within 1 minute of vortexing:

A. Transfer 1 mL of specimen into the APTIMA Specimen Transfer Tube (T652) using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug).

B. Process only 1 ThinPrep and transfer tube set at a time.

C. Recap APTIMA Specimen Transfer Tube tightly and gently invert 3 times to mix.

3. Label APTIMA transfer tube with appropriate label.

4. Use remainder of ThinPrep specimen for Pap testing.

Specimen Stability Information: Transport and store urine specimen transport tube at 2 to 30° C (refrigerate is preferred temperature) within 30 days of collection. If longer storage is needed, freeze at -20 to -70° C for up to 180 days.


Specimen Minimum Volume

The correct volume of urine has been added when the fluid level is between the black fill lines on the urine transport tube.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) APTIMA VIAL
  Frozen  180 days APTIMA VIAL
  Ambient  APTIMA VIAL

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Saturday; Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87661

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TVRNA Trichomonas vaginalis Amplified RNA 46154-1

 

Result ID Test Result Name Result LOINC Value
SRC29 SOURCE: 31208-2
34810 Trichomonas vaginalis amplified RNA 46154-1

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

NY State Approved

Yes

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.